Evaluation of seven different commercially available real-time PCR assays for detection of shiga toxin 1 and 2 gene subtypes.
AuthorsMargot H, Cernela N, Iversen C, Zweifel C, Stephan R,
JournalJ Food Prot
PubMed ID23643131
Following the recent outbreak of Shiga toxin-producing Escherichia coli (STEC) O104:H4 infection in Germany, the demand for fast detection of STEC has again increased. Various real-time PCR-based methods enabling detection of Shiga toxin genes (stx) have been developed and can be used for applications in food microbiology. The present study ... More