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QuantStudio™ 3D Digital PCR Chip Lids and Blades - FAQs

查看更多产品信息 QuantStudio™ 3D Digital PCR Chip Lids and Blades - FAQs (4485510)

14 个常见问题解答

Is there any way to access the raw fluorescence data from the QuantStudio 3D Chips in AnalysisSuite software v3.0?

Yes, there is an option to export the Chip data as .xml on the Export tab in AnalysisSuite software v3.0.

What is the advantage of using the new Poisson Plus algorithm in AnalysisSuite software v3.0?

The Poisson Plus algorithm corrects for well-to-well load volume variation on a per Chip basis. This becomes important at higher target concentrations.

When should I use the new Poisson Plus algorithm in AnalysisSuite software v3.0?

You should use the Poisson Plus algorithm for projects that contain QuantStudio 3D Chips with target quantities >2,000 copies/µL.

Can I purchase Immersion Fluid separately for the QuantStudio 3D Digital PCR System?

Yes, please use Cat. No. A27322.

In the QuantStudio 3D Digital PCR System, how does well-to-well volume variation affect data?

At low concentrations, there is no impact on the data. At higher concentrations (>2,000 copies/µL), there is a slight impact. Using the Poisson Plus algorithm will correct for well-to-well volume variation.

Are there any differences in well-to-well volume variation between the QuantStudio 3D Digital PCR v1 and v2 Chips?

No significant difference has been measured.

Can current QuantStudio 3D Digital PCR v1 Chips be mixed in a project with the new v2 Chips?

Yes, internal studies have shown comparable performance between v1 and v2 Chips.

Can I use QuantStudio 3D Firmware v3.0 to read older Chips/Lids?

Yes, Firmware v3.0 will work with all versions of Chips and Lids.

Can I use QuantStudio 3D Firmware v2.0 or v1.1 to analyze the new v2 Chip?

No. No matter which version of Chip Lid is being used, v2 Chips require Firmware v3.0 for proper data analysis.

Can I use QuantStudio 3D Firmware v2.0 or v1.1 to read the new v2 Chip Lids with 2D barcodes?

We recommend that you upgrade the firmware to v3.0 in order to read the 2D barcode; otherwise the .eds files will not have a Chip ID.

Can I use the QuantStudio 3D Digital PCR Master Mix v2 with the current v1 Chips?

No, the v2 Master Mix can only be used with the v2 Chips.

Can I use the current v1 QuantStudio 3D Digital PCR Master Mix with the new v2 Chips?

No, the current v1 Master Mix can only be used with the older Chips.

I have a lot of the current v1 Applied Biosystems QuantStudio 3D Digital PCR Chips; can they be used with the new v2 Chip Lids?

Yes, the current Chips will work with the new v2 Chip Lids.

I have a lot of the current v1 Applied Biosystems QuantStudio 3D Digital PCR Chip Lids, can they be used with the new v2 Chips?

Yes, the current Lids will work on the new v2 Chips.