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Ion PI™ IC 200 Kit - FAQs

查看更多产品信息 Ion PI™ IC 200 Kit - FAQs (4488377)

11 个常见问题解答

What should I do if the centrifuge is still running even though the time remaining is 0:00 at the final spin of the Ion OneTouch 2?

The "time remaining" is not entirely accurate. The centrifuge may keep rotating for about 15 minutes after the time has become 0:00. If it rotates for more than 15 minutes, please contact Technical Support.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What should I do if an "Over Pressure" error appears after I start an Ion OneTouch 2 run?

The pressure inside the instrument may be above normal. If the Ion OneTouch 2 firmware is older than v540, please update it to the latest version.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are these black precipitates in the recovery tube and waste of the Ion OneTouch System?

It may be rubber from the packing. The amount varies among instruments and it is not anything to be concerned about, as it will be removed by the Ion OneTouch ES.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Which sequencing platform is the Ion PI IC 200 Kit compatible with ?

The Ion PI IC 200 Kit is only compatible with the Ion Chef and Ion Proton sequencing platforms. Components of this kit should not be replaced or interchanged with other product kit components.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How do I update my Ion OneTouch 2 firmware?

Firmware updates to the software controlling the Ion OneTouch 2 Instrument are periodically released. To update the firmware to the appropriate version, use either a USB flash drive or an ethernet connection, as described on page 11 of the Ion OneTouch 2 System - Installation, Setup, and Maintenance User Guide (http://tools.thermofisher.com/content/sfs/manuals/MAN0014388_IonOneTouch2Sys_UG.pdf ).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the currently available Ion OneTouch 2 template kits?

For the Ion PGM System, we offer:
- Ion PGM Hi-Q View OT2 Kit (Cat. No. A29900) for template preparation of libraries up to 400 bp in length.

For the Ion Proton System, we offer:
- Ion PI Hi-Q OT2 200 Kit (Cat. No. A26434) for template preparation of libraries up to 200 bp in length.

For the Ion S5 System and Ion S5 XL System, we offer two kits:
- Ion 520/530 Kit-OT2 (Cat. No. A27751) for template preparation of libraries up to 400 bp in length.
- Ion 540 Kit- OT2 (Cat. No. A27753) for template preparation of libraries up to 200 bp in length.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How do I update my Ion Torrent Ion Proton System to the latest software version?

Firmware updates for Ion instruments are included with the latest version of Ion Torrent Torrent Suite Software. First, download and install the latest version of Torrent Suite Software on your Torrent Server. Once completed, your Ion Proton System should alert you that an update is available. You can also start the update process from the Options menu. From the main menu, select “Options,” then “Updates,” “Released Updates,” and “Check.” Once your Ion Proton System has detected the update, press the “Update” button to begin the process. Instructions to update the Ion Proton Sequencer can also be found in the Appendix of the Ion Proton Sequencing Kit user guides.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I'm getting the "Chip is not ready/Chip Types do not match" error message with my Ion Proton System. What should I do?

After inserting a new chip and prior to pressing the “Next” button, please wait until a green check mark is displayed on the bottom left of the instrument screen. This indicates that the chip is detected.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

I'm getting the "FPGA or CPU temp above threshold" error message with my Ion Proton System. What should I do?

This may indicate a fan failure inside the instrument. Please call 1-87-SEQUENCE to open a service call.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How much oil and recovery solution is typically consumed during a normal Ion Torrent Ion OneTouch 2 run and during a cleaning?

In a typical Ion OneTouch 2 run, you should expect ~18 mm drop in the level of oil in the tube (~22 mL consumed), and ~13 mm drop in the recovery solution (~15 mL consumed). During cleaning, you should expect to collect ~10 mL of oil in the 50 mL conical waste collection tube.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Where can I find the protocols for my Ion Torrent Ion OneTouch 2 kit?

Ion Torrent OneTouch2 protocols can be found at our NGS Support Center (thermofisher.com/ngssupport), in the NGS Template Preparation Support Center section. For all other protocols, please go here (http://www.thermofisher.com/manuals-protocols-search.html).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.