Pierce™ Protein A IgG Plus Orientation Kit, 2 mL - FAQs

What are the Pierce Protein A and Protein G IgG Plus Orientation kits for?

The Orientation kits allow for binding of an antibody to the Protein A or G resin. After the antibody is bound, an amine- reactive crosslinker (DSS) is added to covalently attach the antibody to the support. This allows for the target antigen to elute free of antibody contamination. The Protein A and G Orientation Kits are used for larger-scale purification of IgG than is typical in an immunoprecipitation.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.