Can I immobilize IgY on Protein A, Protein G or Protein A/G to immunoprecipitate or affinity purify the antigen?
No. IgY does not bind to Protein A, Protein G or Protein A/G; however, AminoLink Plus Resin (Cat. No. 20501) or Kit (Cat. No. 44894) can immobilize IgY. The AminoLink Plus Resin is aldehyde-activated and reacts with primary amines on the IgY and forms a covalent linkage.
IgY has a mass of 180kDa, which is more than IgG because the IgY heavy chain has an extra domain on the Fc region. The light chains are ~22 kDa and the heavy chains are ~68 kDa; however, estimates vary in the literature.
How should I store the IgY purified using the Thermo Scientific Chicken IgY Purification Kit??
Store the purified IgY at 0-4°C (< 6 months). For longer storage, separate the IgY into small volumes, add a small amount of glycerol (< 10%) to help stabilize the IgY and store at -20°C.