Pierce™ Protein A Spin Plate for IgG Screening - FAQs

View additional product information for Pierce™ Protein A Spin Plate for IgG Screening - FAQs (45202)

7 product FAQs found

Can I use the Protein A or Protein G Antibody Screening Spin Plate to purify chicken IgY?

No. Neither Protein A nor Protein G binds IgY. Thermo Scientific Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) purifies IgY from serum. The Thermo Scientific Chicken IgY Purification Kits (Cat. Nos. 44918 and 44922) purify IgY from yolks.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I reuse the Protein A and Protein G Antibody Screening Spin Plates?

Yes. To regenerate the resins, wash wells three times with elution buffer and three times with 0.02% sodium azide in ultrapure water as detailed in the instructions.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use just a portion of the Antibody Screening Spin Plate to process a few samples?

No. The Protein A or Protein G 96-well Spin Plates have bottom and top seals. Removing the seals will cause the buffers to leak into the wash/collection plate. It is not possible to remove only a portion of the seal and continue with the purification process, and therefore, process 96 samples each time.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the binding capacities of the Antibody Screening Spin Plates?

The Protein A Spin Plate binds 0.6 mg of human or 0.3-0.4 mg of mouse IgG/well (based on > 12 mg of human IgG or 6-8 mg of mouse IgG/mL of settled resin). The Protein G Spin Plate will bind > 0.5 mg human IgG/well (based on 11-15 mg of human IgG/mL of settled resin).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the difference between Protein A and Protein G binding capabilities?

Protein A and Protein G bind almost exclusively with the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein A is generally preferred for rabbit, pig, dog and cat IgG. Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (IgG1, IgG2, etc.), and it binds rat IgG2a and IgG2b, which do not bind to Protein A. For a comprehensive list of the binding characteristics of Protein A and Protein G, see Tech Tip #34 (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is included with the Antibody Screening Spin Plates?

Each product includes one 96-well Spin Plate and three Wash/Collection plates (buffers are not included). Each of the 96 wells contains 50 µl of agarose resin (200 µl of 25% slurry) with the immobilized protein and can process 10-100 µl of sample.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

In what applications can I use the Antibody Screening Spin Plates?

Protein A (Cat. No. 45202) and Protein G (Cat. No. 45204) Spin Plates for IgG Screening are for rapid, small scale, affinity-purification of polyclonal and monoclonal antibodies from serum, ascites and culture supernatants.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.