Melon 凝胶树脂可与多种物种和亚类的抗体(其中许多使用蛋白 A 或蛋白 G 时无法高效纯化)配合使用。通过该方法分离的 IgG 的所得回收率和纯度与使用结合和释放支持物(如蛋白 A 或蛋白 G)从相同样品中获得的回收率和纯度相当。此外,使用 Melon 凝胶树脂纯化的抗体可回收并立即用于免疫检测或后续偶联或修饰步骤;回收的抗体无需通过中和或脱盐来去除苛刻的洗脱缓冲液。
与传统蛋白 A 或蛋白 G 方法相比,Melon 凝胶 IgG 纯化可节约时间,且不影响抗体的质量。与使用蛋白 A 或蛋白 G 进行的抗体亲和纯化相比,Melon 凝胶 IgG 纯化更简单、更快速,且可为多种抗体物种提供更高的得率和纯度。
描述Melon™ 凝胶 IgG 离心纯化试剂盒,3 mL,离心柱,试剂盒,微量离心机形式,亲和,专有树脂柱类型,具有专有配体固定化相,足以从约 25 x 0.1 mL 血清样品中纯化 IgG
纯化目标IgG 抗体
数量3 mL
色谱柱类型具有专利技术的树脂、亲和性
产品规格试剂盒、微量离心机、离心柱
产品线Melon
产品类型IgG 纯化试剂盒
固定相具有专利技术的配体
Unit SizeEach
内容与储存
足够用于:从约 25 x 0.1 mL 血清样品中纯化 IgG(总计从血清中纯化约 25 mg IgG) • Melon 凝胶支持物,3 mL • Melon 凝胶纯化缓冲液,100 mL • 离心柱,0.8 mL,27 根柱 • 微量离心管,2 mL,30 个管
储存在 4°C 下
常见问题解答 (FAQ)
How can I determine when the Melon Gel Support is saturated?
For serum, a volume double the Melon Gel Support volume is the maximum amount that can be processed at one time. To determine if too much sample was processed, examine the purified IgG by SDS-PAGE for additional protein bands.
No. Thermo Scientific Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) can be used to purify IgY from serum. The Chicken IgY Purification Kit (Cat. No. 44918) can be used to purify IgY from egg yolks.
Is the Melon Gel IgG Spin Purification Kit (Cat. No. 45206) a viable alternative to using traditional Protein A and Protein G methods for purification of IgG from serum?
Yes. With the Melon Gel IgG Spin Purification Kit, 90% recovery of IgG with greater than 80% purity are typically observed for a single purification. Although IgA and IgM may be present along with the purified IgG, a high percentage (> 80%) of IgA and IgM is retained on the gel support. This high binding of IgA and IgM to the gel support combined with the fact that IgA and IgM are present in low abundance in serum results in low levels of these antibody molecules in the purified IgG. Here are other advantages of using the Melon Gel IgG Spin Purification Kit over traditional Protein A or Protein G methods:
-Simple, one-step protocol - IgG purification with Melon Gel Resin does not involve binding, washing, and multiple elution steps required by Protein A and Protein G
-Rapid purification - antibody purification from serum is four to six times faster than Protein A or G methods
-Minimal dilution - reduced need to concentrate antibody before storage or use
-Robust purification - Melon Gel IgG Purification Kits work with a wide range of antibodies including many that do not purify well on Protein A or Protein G
-Gentle purification - No harsh antibody elution conditions helps retain IgG activity
-Regenerable support - Melon Gel Resin can be used for multiple antibody purifications
The Melon Gel Support can be used up to three times. Performing an ammonium sulfate precipitation on serum samples containing large amounts of non-antibody protein before purification improves purification efficiency and increases reusability.