Vertebrate neurogenesis is counteracted by Sox1-3 activity.
AuthorsBylund M, Andersson E, Novitch BG, Muhr J,
JournalNat Neurosci
PubMed ID14517545
'The generation of neurons from stem cells involves the activity of proneural basic helix-loop-helix (bHLH) proteins, but the mechanism by which these proteins irreversibly commit stem cells to neuronal differentiation is not known. Here we report that expression of the transcription factors Sox1, Sox2 and Sox3 (Sox1-3) is a critical ... More
A submicroscopic deletion containing SOX2 was identified at the 3q breakpoint in a child with t(3;11)(q26.3;p11.2) associated with bilateral anophthalmia. Subsequent SOX2 mutation analysis identified de novo truncating mutations of SOX2 in 4 of 35 (11%) individuals with anophthalmia. Both eyes were affected in all cases with an identified mutation. ... More
Cellular characterization of human pluripotent stem cells.
AuthorsQuintanilla RH,
JournalMethods Mol Biol
PubMed ID23546756
Human pluripotent stem cells (PSCs), in particular induced PSCs, are very difficult to derive, grow, and bank. They require extensive amounts of resources and time to render them useful for basic and applied research. As the derivation methods, culture systems and tissues of origin differ, so does the quality of ... More
The HMG-box transcription factor SoxNeuro acts with Tcf to control Wg/Wnt signaling activity.
AuthorsChao AT, Jones WM, Bejsovec A,
JournalDevelopment
PubMed ID17267442
Wnt signaling specifies cell fates in many tissues during vertebrate and invertebrate embryogenesis. To understand better how Wnt signaling is regulated during development, we have performed genetic screens to isolate mutations that suppress or enhance mutations in the fly Wnt homolog, wingless (wg). We find that loss-of-function mutations in the ... More
SOX2 is a dose-dependent regulator of retinal neural progenitor competence.
AuthorsTaranova OV, Magness ST, Fagan BM, Wu Y, Surzenko N, Hutton SR, Pevny LH,
JournalGenes Dev
PubMed ID16651659
Approximately 10% of humans with anophthalmia (absent eye) or severe microphthalmia (small eye) show haploid insufficiency due to mutations in SOX2, a SOXB1-HMG box transcription factor. However, at present, the molecular or cellular mechanisms responsible for these conditions are poorly understood. Here, we directly assessed the requirement for SOX2 during ... More