Global Expression Profiling of Acetate-grown Escherichia coli.
Authors Oh Min-Kyu; Rohlin Lars; Kao Katy C; Liao James C;
JournalJ Biol Chem
PubMed ID11815613
'This study characterized the transcript profile of Escherichia coli in acetate cultures using DNA microarray on glass slides. Glucose-grown cultures were used as a reference. At the 95% confidence level, 354 genes were up-regulated in acetate, while 370 genes were down-regulated compared with the glucose-grown culture. Generally, more metabolic genes ... More
RasGRP, a Ras guanyl nucleotide- releasing protein with calcium- and diacylglycerol-binding motifs.
AuthorsEbinu JO, Bottorff DA, Chan EY, Stang SL, Dunn RJ, Stone JC
JournalScience
PubMed ID9582122
RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. Besides the catalytic domain, RasGRP has an atypical pair of EF hands that bind calcium and a diacylglycerol (DAG)-binding domain. RasGRP activated Ras and caused transformation in fibroblasts. A DAG analog caused sustained activation of Ras-Erk signaling ... More
Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy.
AuthorsZhang CL, McKinsey TA, Chang S, Antos CL, Hill JA, Olson EN,
JournalCell
PubMed ID12202037
The heart responds to stress signals by hypertrophic growth, which is accompanied by activation of the MEF2 transcription factor and reprogramming of cardiac gene expression. We show here that class II histone deacetylases (HDACs), which repress MEF2 activity, are substrates for a stress-responsive kinase specific for conserved serines that regulate ... More
Improvements in siRNA properties mediated by 2'-deoxy-2'-fluoro-beta-D-arabinonucleic acid (FANA).
AuthorsDowler T, Bergeron D, Tedeschi AL, Paquet L, Ferrari N, Damha MJ,
JournalNucleic Acids Res
PubMed ID16554553
RNA interference (RNAi) has emerged recently as an efficient mechanism for specific gene silencing. Short double-stranded small interfering RNAs (siRNAs) are now widely used for cellular or drug target validation; however, their use for silencing clinically relevant genes in a therapeutic setting remains problematic because of their unfavourable metabolic stability ... More
Use of an in vivo reporter assay to test for transcriptional and translational fidelity in yeast.
Authors Shaw Randal J; Bonawitz Nicholas D; Reines Daniel;
JournalJ Biol Chem
PubMed ID12006589
Eukaryotic RNA polymerase II and Escherichia coli RNA polymerase possess an intrinsic ribonuclease activity that is stimulated by the polymerase-binding proteins SII and GreB, respectively. This factor-activated hydrolysis of nascent RNA has been postulated to be involved in transcription elongation as well as removal of incorrect bases misincorporated into RNA. ... More
Genomewide identification of Pseudomonas syringae pv. tomato DC3000 promoters controlled by the HrpL alternative sigma factor.
Authors Fouts Derrick E; Abramovitch Robert B; Alfano James R; Baldo Angela M; Buell C Robin; Cartinhour Samuel; Chatterjee Arun K; D'Ascenzo Mark; Gwinn Michelle L; Lazarowitz Sondra G; Lin Nai-Chun; Martin Gregory B; Rehm Amos H; Schneider David J; van Dijk Karin; Tang Xiaoyan; Collmer Alan;
JournalProc Natl Acad Sci U S A
PubMed ID11854524
The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize tomato and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses of DC3000, and specifically, to identify genes involved in pathogenesis, we developed a draft sequence of DC3000 and used an ... More