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Invitrogen
Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 660, eBioscience™
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产品信息
50-4888-80
应用
建议稀释比
免疫组化 (IHC)
Assay-Dependent
免疫细胞化学 (ICC/IF)
Assay-Dependent
流式细胞分析 (Flow)
Assay-Dependent
对照试剂 (Ctrl)
Assay-Dependent
产品规格
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Isotype Control
克隆号
eBio299Arm
偶联物
eFluor™ 660
eFluor™ 660
eFluor™ 660
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
规格
25 µg
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.
Applications Reported: This eBio299Arm antibody has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.
Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use isotype control at the same concentration as experimental antibody.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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