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Ki-67 Monoclonal Antibody (20Raj1), eFluor™ 660, eBioscience™
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图: 1 / 18
Ki-67 Antibody (50-5699-80) in ICC/IF
Immunofluorescence analysis of Ki-67 Monoclonal Antibody (20Raj1), eFluor™ 660, eBioscience™ was performed using 70% confluent log phase HeLa cells serum starved (SS) for 36 hours followed by serum release (SR) for 6 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (20Raj1), eFluor™ 660, eBioscience™ (Product # 50-5699-82) at 5 µg/mL concentration in 0.1%... View More
产品信息
50-5699-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
20Raj1
偶联物
eFluor™ 660
eFluor™ 660
eFluor™ 660
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
规格
25 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein. Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells. Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0). During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3). In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells.
This monoclonal antibody 20Raj1 recognizes canine Ki-67.
Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This 20Raj1 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized HeLa cells, immunohistochemistry on FFPE human skin using low pH antigen retrieval, and flow cytometry on stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. For immunocytochemistry immunohistochemistry, this can be used at less than or equal to 20 µg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.125 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; antigen Ki67; Molecular Immunology Borstel antibody 1; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; unnamed protein product
基因别名: KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013
Entrez Gene ID: (Dog) 100686578, (Human) 4288
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