Invitrogen

Aiolos Monoclonal Antibody (8B2), eFluor™ 660, eBioscience™

Name: Aiolos Monoclonal Antibody (8B2), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-5789-82

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1 篇参考文献

查看其他20个Aiolos (IKZF3)抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
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Aiolos Antibody in Flow Cytometry (Flow)

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Aiolos Antibody (50-5789-82) in Flow

Surface staining of C57Bl/6 splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) followed by intracellular staining with 0.5 µg of Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or 0.5 µg of Anti-Mouse Aiolos eFluor® 660 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Total cells were used for analysis.

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产品信息

50-5789-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

1 µg/test

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

8B2

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_11217888

产品详细信息

Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors. Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells. Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins. Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells. Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression. Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling.

Applications Reported: This 8B2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 8B2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. At least six alternative transcripts encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。