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Invitrogen
T-bet Monoclonal Antibody (eBio4B10 (4B10)), eFluor™ 660, eBioscience™
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T-bet Antibody (50-5825-80) in Flow
Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and 0.25 µg of Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or 0.25 µg of Anti-Human/Mouse T-bet eFluor® 660 (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.
产品信息
50-5825-80
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rhesus monkey
已发表种属
Hamster,
Human,
Mouse
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBio4B10 (4B10)
偶联物
eFluor™ 660
eFluor™ 660
eFluor™ 660
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
规格
25 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The eBio4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
It is recommended to use the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to BestProtocols® for Staining Protocol (Protocol B: One step protocol for intracellular (nuclear) proteins)
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor expressed in T cells; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM
基因别名: IMD88; T-bet; T-PET; TBET; TBLYM; TBT1; TBX21
UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8
Entrez Gene ID: (Human) 30009, (Rhesus monkey) 694044, (Mouse) 57765
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription regulatory region DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell fate specification
T-helper 1 cell lineage commitment
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to virus
positive regulation of gene expression
T cell differentiation
negative regulation of interleukin-2 production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of T cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
regulation of immune response
lymphocyte migration
negative regulation of T-helper 17 cell differentiation
negative regulation of T-helper 17 cell lineage commitment
negative regulation of T-helper 2 cell cytokine production
positive regulation of T-helper 1 cell cytokine production
transcription, DNA-templated
cellular response to organic substance
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