Invitrogen

c-Rel Monoclonal Antibody (1RELAH5), eFluor™ 660, eBioscience™

Name: c-Rel Monoclonal Antibody (1RELAH5), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-6111-80

3个已发表图片

5 篇参考文献

查看其他27个c-Rel抗体

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (1)
已发表图片 (3)

图: 1 / 4

c-Rel Antibody (50-6111-80) in Flow

Mouse thymocytes were stained with Anti-Mouse CD4, Anti-Mouse CD8 and Anti-Mouse CD25, then fixed and permeabilized with Foxp3 Staining Buffer, and stained intracellularly for Anti-Mouse Foxp3 and Anti-Mouse c-Rel eFluor® 660. Anti-Mouse cRel eFluor® 660 staining (right) was compared in CD8+CD4. (left, green), CD4+CD8+ (left, blue), CD4+CD8-CD25-Foxp3. (center, orange), and CD4+CD8-CD25+Foxp3+ (center, pink) populations.

c-Rel Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

产品信息

50-6111-80

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.06 µg/test

查看3篇已发表文章 3篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1RELAH5

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

25 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574260

产品详细信息

Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.

Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).

仅用于科研。不用于诊断过程。未经明确授权不得转售。