Invitrogen

Synaptophysin Monoclonal Antibody (EP10), eFluor™ 660, eBioscience™

Name: Synaptophysin Monoclonal Antibody (EP10), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-6525-82

高级验证

2个已发表图片

3 篇参考文献

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说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

说明书

证书

实验方案

问题与答案

技术支持

Additional Information:

Synaptophysin Antibody in Immunocytochemistry (ICC/IF)

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Synaptophysin Antibody (50-6525-82) in ICC/IF

Immunofluorescence analysis of Synaptophysin was performed using SH-SY5Y cells differentiated into neurons. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Synaptophysin Monoclonal Antibody (EP10), eFluor 660, eBioscience™ (Product # 50-6525-82) at 5 µg/mL i... View More

Synaptophysin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Synaptophysin Antibody in Immunohistochemistry (IHC)

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产品信息

50-6525-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

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产品规格

种属反应

Hamster, Human

已发表种属

Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

EP10

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574271

产品详细信息

Description: This EP10 monoclonal antibody reacts with human Synaptophysin, which is also known as Major Synaptic Vesicle Protein p38. This 38-kDa integral membrane protein is expressed on neuronal presynaptic vesicles in the brain, spinal cord, retina, neuromuscular junctions, and adrenal medulla. Synaptophysin is also expressed in neuroendocrine tumors and neuroblastomas. Synaptophysin has been reported to play a role in synaptic vesicle trafficking.

The EP10 antibody recognizes human and hamster synaptophysin but does not recognize mouse and rat synaptophysin.

Applications Reported: This EP10 antibody has been reported for use in immunohistochemical staining, and immunocytochemistry.

Applications Tested: This EP10 antibody has been tested by immunohistochemistry on FFPE human tissue using low pH antigen retrieval at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Synaptophysin is a calcium-binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons. Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis. It is also involved in the regulation of short-term and long-term synaptic plasticity. Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors. Mutations in the gene can result in mental retardation, X-linked 96.

仅用于科研。不用于诊断过程。未经明确授权不得转售。