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Invitrogen
IFN gamma Monoclonal Antibody (XMG1.2), eFluor™ 660, eBioscience™
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IFN gamma Antibody (50-7311-82) in Flow
Intracellular staining of Con A-stimulated C57Bl/6 splenocytes with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.25 µg of Rat IgG1 K Isotype Control eFluor® 660 (Product # 50-4301-82) (left) or 0.25 µg of Anti-Mouse IFN gamma eFluor® 660 (right) using the Fixation and Permeabilization Buffers (Product # 88-8823-88). Total viable cells were used for analysis.
产品信息
50-7311-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
XMG1.2
偶联物
eFluor™ 660
eFluor™ 660
eFluor™ 660
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.
Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: gamma interferon; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g
基因别名: Ifg; IFN-g; Ifng
UniProt ID: (Mouse) P01580
Entrez Gene ID: (Mouse) 15978
protein import into nucleus, translocation
negative regulation of transcription from RNA polymerase II promoter
neutrophil apoptotic process
regulation of the force of heart contraction
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
regulation of transcription, DNA-templated
apoptotic process
inflammatory cell apoptotic process
immune response
humoral immune response
cell cycle arrest
positive regulation of cell proliferation
negative regulation of cell proliferation
response to virus
positive regulation of epithelial cell migration
antigen processing and presentation
neutrophil chemotaxis
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of synaptic transmission, cholinergic
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of tumor necrosis factor production
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
regulation of growth
positive regulation of T cell proliferation
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
negative regulation of growth of symbiont involved in interaction with host
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
positive regulation of cell adhesion
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
negative regulation of smooth muscle cell proliferation
positive regulation of interleukin-1 beta secretion
regulation of immune response
regulation of insulin secretion
sensory perception of mechanical stimulus
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
cellular response to lipopolysaccharide
cellular response to interleukin-18
extrinsic apoptotic signaling pathway
regulation of neuronal action potential
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
regulation of hepatocyte proliferation
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