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IL-17FF (homodimer) Monoclonal Antibody (WU24A4P), eFluor™ 660, eBioscience™
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FIGURE: 1 / 1
IL-17FF (homodimer) Antibody (50-9178-42) in Flow
Th17-polarized normal human peripheral blood cells were treated with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975) and then intracellulary stained with Anti-Human IL-17A FITC (Product # 11-7179) and Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732) (left) or Anti-Human IL-17FF (homodimer) eFluor® 660 (right) using the Intracellular Fixation and Permeabiliz... View More
Product Details
50-9178-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
WU24A4P
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The WU24A4P monoclonal antibody reacts with human interleukin (IL)-17F homodimer (IL-17FF). This antibody specifically reacts with this homodimer and has no reactivity for the IL-17AF heterodimer. IL-17FF is a 34 kDa member of the IL-17 family, which includes IL-17A, B, C, D, and E (IL-25). Within this family, IL-17A and IL-17F are produced preferentially by T helper 17 (Th17) cells, with other members produced more widely. IL-17F and IL-17A have many overlapping functions, but knockout studies have shown each to have independent functions as well. In vitro cultured Th17 cells have been shown to express IL-17FF and IL-17AA homodimers and IL-17AF heterodimers depending on the culture conditions and their differentiation state. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
While other clones with specificity for IL-17F, such as SHLR17, react with the IL-17F monomer in the context both IL-17FF and IL-17AF dimers, clone WU24A4P reacts specifically with IL-17FF homodimer and does not detect IL-17AF heterodimer.
Applications Reported: This WU24A4P antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This WU24A4P antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Th17-polarized normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 17F is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflamation by increasing chemokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; Cytokine ML-1; IL-17F; ILN; Interleukin; Interleukin-17F
Gene Aliases: CANDF6; IL-17F; IL17A; IL17F; ML-1; ML1
UniProt ID: (Human) Q96PD4
Entrez Gene ID: (Human) 112744
positive regulation of cytokine production
positive regulation of antimicrobial peptide production
adaptive immune response
immune system process
inflammatory response
negative regulation of angiogenesis
regulation of transforming growth factor beta receptor signaling pathway
regulation of granulocyte macrophage colony-stimulating factor production
regulation of interleukin-2 production
regulation of interleukin-6 production
regulation of interleukin-8 production
positive regulation of interleukin-6 production
positive regulation of lymphotoxin A production
innate immune response
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
cartilage development
regulation of primary metabolic process
interleukin-17-mediated signaling pathway
positive regulation of cytokine production involved in inflammatory response
positive regulation of tumor necrosis factor superfamily cytokine production
positive regulation of chemokine (C-X-C motif) ligand 1 production
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