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S100A8 Monoclonal Antibody (CF-145), eFluor™ 660, eBioscience™
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FIGURE: 1 / 2
S100A8 Antibody (50-9745-42) in Flow
Normal human peripheral blood cells were incubated with Human Fc Receptor Binding Inhibitor Purified (Product # 14-9161-73) for 20 minutes, then stained with Anti-Human CD11b PE (Product # 12-0118-42) followed by fixation and permeabilization with the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. The cells were then stained with Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or Anti-Human S100A8 eFluor® 660 (right). Cells in the monocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
50-9745-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
CF-145
Conjugate
eFluor™ 660
eFluor™ 660
eFluor™ 660
Excitation/Emission Max
651/668 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody CF-145 recognizes human S100A8. S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8) or Calgranulin-A, is a calcium binding protein and member of the S100 family of proteins. S100A8 is expressed by neutrophils, monocytes, macrophages, granulocytes, and epithelial cells during dermatoses, such as psoriasis. S100A8 forms a heterodimer with S100A9, which together is known as calprotectin. S100A8 has antimicrobial and pro-apoptotic properties and is a pro-inflammatory mediator in acute and chronic inflammation. Levels of S100A8 are elevated in patients with various inflammatory disorders such as cystic fibrosis, rheumatoid arthritis, and chronic bronchitis. Upregulation of S100A8 is also observed in many tumors, including skin, breast, colon, pancreatic, and ovarian cancers.
Applications Reported: This CF-145 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.
Applications Tested: This CF-145 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: calgranulin A; calprotectin L1L subunit; cystic fibrosis antigen; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 A8; S100A8; unnamed protein product; urinary stone protein band A
Gene Aliases: 60B8AG; CAGA; CFAG; CGLA; CP-10; L1Ag; MA387; MRP8; NIF; P8; S100-A8
UniProt ID: (Human) P05109
Entrez Gene ID: (Human) 6279
leukocyte migration involved in inflammatory response
chronic inflammatory response
autophagy
inflammatory response
response to zinc ion
peptidyl-cysteine S-nitrosylation
positive regulation of cell growth
neutrophil chemotaxis
response to lipopolysaccharide
regulation of toll-like receptor signaling pathway
autocrine signaling
defense response to bacterium
endothelial cell migration
response to ethanol
positive regulation of inflammatory response
defense response to fungus
regulation of cytoskeleton organization
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
Disclaimer
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