The human aminopeptidase N gene: isolation, chromosome localization, and DNA polymorphism analysis.
AuthorsWatt VM, Willard HF
JournalHum Genet
PubMed ID1977688
'DNA encoding the human aminopeptidase N (EC 3.4.11.2) gene (PEPN) was first isolated using rat cDNA probes and then used in Southern analysis of DNA from mouse-human somatic cell hybrids to assign this gene to the long-arm region (q11-qter) of human chromosome 15. This human genomic DNA probe detects a ... More
Human aminopeptidase N is a receptor for human coronavirus 229E.
Human coronaviruses (HCV) in two serogroups represented by HCV-229E and HCV-OC43 are an important cause of upper respiratory tract infections. Here we report that human aminopeptidase N, a cell-surface metalloprotease on intestinal, lung and kidney epithelial cells, is a receptor for human coronavirus strain HCV-229E, but not for HCV-OC43. A ... More
Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N.
AuthorsLook AT, Ashmun RA, Shapiro LH, Peiper SC
JournalJ Clin Invest
PubMed ID2564851
To determine the primary structure of CD13, a 150-kD cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells, we isolated the complete sequences encoding the polypeptide in overlapping complementary DNA (cDNA) clones. The authenticity of our cDNA clones was demonstrated by the ability of the ... More
Separate promoters control transcription of the human aminopeptidase N gene in myeloid and intestinal epithelial cells.
AuthorsShapiro LH, Ashmun RA, Roberts WM, Look AT
JournalJ Biol Chem
PubMed ID1675638
Aminopeptidase N is a membrane-bound metalloprotease expressed on the surface of normal and malignant human myeloid cells, fibroblasts, hepatocytes, and the epithelial cells that form brush borders of the small intestine and kidney. Northern blot analysis of RNA extracted from these tissues revealed two distinct aminopeptidase N transcripts: a 3.7-kilobase ... More