Detection protocols for biotinylated probes: optimization using multistep techniques.
AuthorsMcQuaid S, Allan GM
JournalJ Histochem Cytochem
PubMed ID1552190
Recent studies using biotinylated in situ hybridization (ISH) have utilized a wide range of detection protocols for the biotinylated hybrids, leading to conflicting reports in the literature regarding sensitivity. In this study we compared 11 different detection protocols for biotinylated ISH using a measles virus-specific RNA probe on formalin-fixed, paraffin-embedded ... More