Multisite M-phase phosphorylation of Xenopus Wee1A.
AuthorsKim SY, Song EJ, Lee KJ, Ferrell JE,
JournalMol Cell Biol
PubMed ID16287869
The Cdk1 inhibitor Wee1 is inactivated during mitotic entry by proteolysis, translational regulation, and transcriptional regulation. Wee1 is also regulated by posttranslational modifications, and here we have identified five phosphorylation sites in the N-terminal domain of embryonic Xenopus Wee1A through a combination of mutagenesis studies and matrix-assisted laser desorption ionization-time ... More
Building a cell cycle oscillator: hysteresis and bistability in the activation of Cdc2.
AuthorsPomerening JR, Sontag ED, Ferrell JE,
JournalNat Cell Biol
PubMed ID12629549
In the early embryonic cell cycle, Cdc2-cyclin B functions like an autonomous oscillator, whose robust biochemical rhythm continues even when DNA replication or mitosis is blocked. At the core of the oscillator is a negative feedback loop; cyclins accumulate and produce active mitotic Cdc2-cyclin B; Cdc2 activates the anaphase-promoting complex ... More
Dissolution of the maskin-eIF4E complex by cytoplasmic polyadenylation and poly(A)-binding protein controls cyclin B1 mRNA translation and oocyte maturation.
AuthorsCao Q, Richter JD,
JournalEMBO J
PubMed ID12110596
Cytoplasmic polyadenylation stimulates the translation of several dormant mRNAs during oocyte maturation in Xenopus. Polyadenylation is regulated by the cytoplasmic polyadenylation element (CPE), a cis-acting element in the 3'-untranslated region of responding mRNAs, and its associated factor CPEB. CPEB also binds maskin, a protein that in turn interacts with eIF4E, ... More