One nucleotide in a kappaB site can determine cofactor specificity for NF-kappaB dimers.
AuthorsLeung TH, Hoffmann A, Baltimore D,
JournalCell
PubMed ID15315758
'The transcription factor NF-kappaB regulates a wide variety of genes involved in multiple processes. Although the apparent consensus sequence of DNA binding sites for NF-kappaB (kappaB sites) is very broad, the sites active in any one gene show remarkable evolutionary stability. Using a lentivirus-based methodology for implantation of gene regulatory ... More
Type I interferon production enhances susceptibility to Listeria monocytogenes infection.
AuthorsO'Connell RM, Saha SK, Vaidya SA, Bruhn KW, Miranda GA, Zarnegar B, Perry AK, Nguyen BO, Lane TF, Taniguchi T, Miller JF, Cheng G,
JournalJ Exp Med
PubMed ID15302901
'Numerous bacterial products such as lipopolysaccharide potently induce type I interferons (IFNs); however, the contribution of this innate response to host defense against bacterial infection remains unclear. Although mice deficient in either IFN regulatory factor (IRF)3 or the type I IFN receptor (IFNAR)1 are highly susceptible to viral infection, we ... More
The leader protein of Theiler's virus interferes with nucleocytoplasmic trafficking of cellular proteins.
AuthorsDelhaye S, van Pesch V, Michiels T,
JournalJ Virol
PubMed ID15047849
'The leader protein of Theiler''s virus was previously shown to block the production of alpha/beta interferon by infected cells. Here, we observed that expression of the leader protein in infected cells triggered subcellular redistribution of a nucleus-target green fluorescent protein. It enhanced redistribution of the nuclear polypyrimidine tract-binding protein but ... More
Protein kinase IKKß-catalyzed phosphorylation of IRF5 at Ser462 induces its dimerization and nuclear translocation in myeloid cells.
AuthorsLopez-Pelaez M, Lamont DJ, Peggie M, Shpiro N, Gray NS, Cohen P,
Journal
PubMed ID25326418
The siRNA knockdown of IFN Regulatory Factor 5 (IRF5) in the human plasmacytoid dendritic cell line Gen2.2 prevented IFNß production induced by compound CL097, a ligand for Toll-like receptor 7 (TLR7). CL097 also stimulated the phosphorylation of IRF5 at Ser462 and stimulated the nuclear translocation of wild-type IRF5, but not ... More
Host specificity of the anti-interferon and anti-apoptosis activities of parainfluenza virus P/C gene products.
AuthorsChambers R, Takimoto T,
JournalJ Gen Virol
PubMed ID19423549
Human parainfluenza virus type 1 (HPIV-1) and Sendai virus (SeV) are highly homologous in structure and sequence, whilst maintaining distinct host ranges. These viruses express accessory proteins from their P/C gene that are known to have activities against innate immunity. The accessory proteins expressed from the P/C gene of these ... More
Differential requirement for TANK-binding kinase-1 in type I interferon responses to toll-like receptor activation and viral infection.
TANK-binding kinase-1 (TBK1) and the inducible IkappaB kinase (IKK-i) have been shown recently to activate interferon (IFN) regulatory factor-3 (IRF3), the primary transcription factor regulating induction of type I IFNs. Here, we have compared the role and specificity of TBK1 in the type I IFN response to lipopolysaccharide (LPS), polyI:C, ... More