High Five™ Cells in Express Five™ Medium

High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichoplusia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). High Five™ Insect Cells are adapted to serum-free culture in Express Five™ SFM (Cat. No. 10486025) for maximum cell growth and recombinant gene expression.

High Five™ Insect Cells (frozen in Express Five™ SFM) features:

• Recombinant protein expression from a variety of expression systems
• Up to ten-fold increase in secreted protein expression compared to Sf9 cells
• Good growth in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression from a variety of expression systems

High levels of protein expression in High Five™ cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac™ Baculovirus Expression System (Cat. No. K81001), or the InsectDirect™ Expression System (Cat. No. K81001).

Up to ten-fold increase in secreted protein expression compared to Sf9 cells

High Five™ cells are the best choice for expression of secreted protein in insect cells. They consistently express 5–10 times more protein that Sf9 cells.

Good growth in adherent or suspension culture

High Five ™ cells thaw into adherent culture, forming an irregular monolayer with plaques that may be difficult to isolate. Protocols for adherent growth can be found in Express Five™ SFM (Cat. No. 10486025) are available in the product manual. High Five™ cells can be adapted to suspension culture.

Quality and performance testing

Each lot of High Five ™ cells is tested for cell growth and viability post-recovery from cryopreservation.

Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling.