Functional characterization and localization of a gill-specific claudin isoform in Atlantic salmon.
AuthorsEngelund MB, Yu AS, Li J, Madsen SS, Færgeman NJ, Tipsmark CK
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID21975646
'Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is associated with remodeling of the epithelium during salinity change. This ... More
Claudin-8 expression in Madin-Darby canine kidney cells augments the paracellular barrier to cation permeation.
'Claudins are a family of integral membrane proteins of the tight junction that are thought to participate in the permeation of solutes across epithelia via the paracellular pathway. Claudin-8 is expressed in the distal renal tubule, which has a characteristically low passive permeability to monovalent cations. To test the hypothesis ... More
Amphiregulin causes functional downregulation of adherens junctions in psoriasis.
AuthorsChung E, Cook PW, Parkos CA, Park YK, Pittelkow MR, Coffey RJ,
JournalJ Invest Dermatol
PubMed ID15955087
'Overexpression of amphiregulin (AR) has been linked to psoriasis in mouse and man. Since psoriasis is marked by hyperproliferation of keratinocytes and loss of epidermal barrier function with infiltration of inflammatory cells into the epidermis and dermis, we hypothesized that AR might contribute to the pathogenesis of psoriasis by affecting ... More
Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells.
AuthorsGuo X, Rao JN, Liu L, Zou T, Keledjian KM, Boneva D, Marasa BS, Wang JY,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15691870
'Occludin is an integral membrane protein that forms the sealing element of tight junctions and is critical for epithelial barrier function. Polyamines are implicated in multiple signaling pathways driving different biological functions of intestinal epithelial cells (IEC). The present study determined whether polyamines are involved in expression of occludin and ... More
Dynamics of claudins expression in colitis and colitis-associated cancer in rat.
AuthorsArimura Y, Nagaishi K, Hosokawa M,
JournalMethods Mol Biol
PubMed ID21717373
'Claudins comprise a multigene family of 24 species and have been shown to constitute the backbone of tight junction strands in simple epithelial cells and to be directly involved in their barrier functions. Apical-most tight junction protein complexes (TJs) are implicated in inflammatory bowel disease (IBD) pathophysiology. Except for claudin-8, ... More
Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.
'Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are ... More
Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium.
AuthorsAcharya P, Beckel J, Ruiz WG, Wang E, Rojas R, Birder L, Apodaca G,
JournalAm J Physiol Renal Physiol
PubMed ID15068973
'In mammals, the bladder stores urine without permitting the passage of urine contents into the bloodstream, a function, in part, of the uroepithelial-associated tight junction complex. The protein constituents that make up this high-resistance barrier in the bladder are currently unknown, although the claudins, a multigene family, are thought to ... More
Extracellular signal-regulated kinases 1/2 control claudin-2 expression in Madin-Darby canine kidney strain I and II cells.
AuthorsLipschutz JH, Li S, Arisco A, Balkovetz DF,
JournalJ Biol Chem
PubMed ID15569684
'The tight junction of the epithelial cell determines the characteristics of paracellular permeability across epithelium. Recent work points toward the claudin family of tight junction proteins as leading candidates for the molecular components that regulate paracellular permeability properties in epithelial tissues. Madin-Darby canine kidney (MDCK) strain I and II cells ... More
Distribution of tight junction proteins in adult human salivary glands.
AuthorsMaria OM, Kim JW, Gerstenhaber JA, Baum BJ, Tran SD
JournalJ Histochem Cytochem
PubMed ID18765838
'Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to ... More
Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins.
AuthorsCarrozzino F, Soulié P, Huber D, Mensi N, Orci L, Cano A, Féraille E, Montesano R,
JournalAm J Physiol Cell Physiol
PubMed ID15930145
Constitutive expression of the transcription factor Snail was previously shown to trigger complete epithelial-mesenchymal transition (EMT). The aim of this study was to determine whether inducible expression of Snail could modify epithelial properties without eliciting full mesenchymal conversion. For this purpose, we expressed mouse Snail (mSnail) cDNA in Madin-Darby canine ... More
Epidermal growth factor receptor activation differentially regulates claudin expression and enhances transepithelial resistance in Madin-Darby canine kidney cells.
AuthorsSingh AB, Harris RC,
JournalJ Biol Chem
PubMed ID14593119
Tight junctions (TJs) are the most apical cell-cell junctions, and claudins, the recently identified TJ proteins, are critical for maintaining cell-cell adhesion in epithelial cell sheets. Based on their in vivo distribution and the results of overexpression studies, certain claudins, including claudin-1 and -4, are postulated to increase, whereas other ... More
Inflammatory processes have differential effects on claudins 2, 3 and 4 in colonic epithelial cells.
AuthorsPrasad S, Mingrino R, Kaukinen K, Hayes KL, Powell RM, MacDonald TT, Collins JE,
JournalLab Invest
PubMed ID16007110
Claudin proteins comprise a recently described family of tight junction proteins that differentially regulate paracellular permeability. Since other tight junction proteins show alterations in distribution or expression in inflammatory bowel disease (IBD) we assessed expression of claudins (CL) 2, 3 and 4 in IBD. CL 2 was strongly expressed along ... More
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells.
AuthorsAmasheh S, Meiri N, Gitter AH, Schöneberg T, Mankertz J, Schulzke JD, Fromm M,
JournalJ Cell Sci
PubMed ID12432083
Tight junctions seal the paracellular pathway of epithelia but, in leaky tissues, also exhibit specific permeability. In order to characterize the contribution of claudin-2 to barrier and permeability properties of the tight junction in detail, we studied two strains of Madin-Darby canine kidney cells (MDCK-C7 and MDCK-C11) with different tight ... More
Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms.
AuthorsMcLaughlin J, Padfield PJ, Burt JP, O'Neill CA,
JournalAm J Physiol Cell Physiol
PubMed ID15229101
On interaction with the intestine, the mycotoxin ochratoxin A is know to cause rapid inflammation, diarrhea, and increased bacterial translocation. All these effects are consistent with a decrease in epithelial barrier function. However, this has not been shown directly. We determined that ochratoxin A is able to reduce the barrier ... More
Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.
AuthorsKratzer I, Vasiljevic A, Rey C, Fevre-Montange M, Saunders N, Strazielle N, Ghersi-Egea JF,
JournalHistochem Cell Biol
PubMed ID22886143
The choroid plexus epithelium controls the movement of solutes between the blood and the cerebrospinal fluid. It has been considered as a functionally more immature interface during brain development than in adult. The anatomical basis of this barrier is the interepithelial choroidal junction whose tightness has been attributed to the ... More
Effects of proinflammatory cytokines on the claudin-19 rich tight junctions of human retinal pigment epithelium.
AuthorsPeng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ,
JournalInvest Ophthalmol Vis Sci
PubMed ID22761260
Chronic, subclinical inflammation contributes to the pathogenesis of several ocular diseases, including age-related macular degeneration. Proinflammatory cytokines affect tight junctions in epithelia that lack claudin-19, but in the retinal pigment epithelium claudin-19 predominates. We examined the effects of cytokines on the tight junctions of human fetal RPE (hfRPE). hfRPE was ... More
Paracellular Cl- permeability is regulated by WNK4 kinase: insight into normal physiology and hypertension.
AuthorsKahle KT, Macgregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP,
JournalProc Natl Acad Sci U S A
PubMed ID15465913
Paracellular ion flux across epithelia occurs through selective and regulated pores in tight junctions; this process is poorly understood. Mutations in the kinase WNK4 cause pseudohypoaldosteronism type II (PHAII), a disease featuring hypertension and hyperkalemia. Whereas WNK4 is known to regulate several transcellular transporters and channels involved in NaCl and ... More
Cultured monolayers of the dog jejunum with the structural and functional properties resembling the normal epithelium.
AuthorsWeng XH, Beyenbach KW, Quaroni A,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15550553
The development of a culture of the normal mammalian jejunum motivated this work. Isolated crypt cells of the dog jejunum were induced to form primary cultures on Snapwell filters. Up to seven subcultures were studied under the electron microscope and in Ussing chambers. Epithelial markers were identified by RT-PCR, Western ... More
Transmigration of neural stem cells across the blood brain barrier induced by glioma cells.
AuthorsDíaz-Coránguez M, Segovia J, López-Ornelas A, Puerta-Guardo H, Ludert J, Chávez B, Meraz-Cruz N, González-Mariscal L,
JournalPLoS One
PubMed ID23637756
Transit of human neural stem cells, ReNcell CX, through the blood brain barrier (BBB) was evaluated in an in vitro model of BBB and in nude mice. The BBB model was based on rat brain microvascular endothelial cells (RBMECs) cultured on Millicell inserts bathed from the basolateral side with conditioned ... More
Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells.
AuthorsPoliak S, Matlis S, Ullmer C, Scherer SS, Peles E
JournalJ Cell Biol
PubMed ID12403818
The apposed membranes of myelinating Schwann cells are joined by several types of junctional specializations known as autotypic or reflexive junctions. These include tight, gap, and adherens junctions, all of which are found in regions of noncompact myelin: the paranodal loops, incisures of Schmidt-Lanterman, and mesaxons. The molecular components of ... More