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查看更多产品信息 BindIt™ Software, With CD only - FAQs (5189009)
14 个常见问题解答
是的，MagMAX Express-96仪器可以和装有最新版本BindIt软件v3.3.1.7的电脑相连。这种连接需要一个到USB转化器的串行端口。已有的.kf2 文件在BindIt软件内不能被编辑，但是可以将.kf2文件重写为.bdz文件从而可以被编辑。请参见应用注意（https://tools.thermofisher.com/content/sfs/brochures/KingFisher-BindIT-TechNote-5189009.pdf）了解更多细节。
BindIt软件和Windows XP (Professional with Service Pack 3)，Windows Vista (Service Pack 2), Windows 7 (Service Pack 1), 以及Windows 8兼容。
Please follow these steps to load a script from your computer to the KingFisher Flex or KingFisher Duo/Duo Prime instrument:
1. Use the USB cable to connect the PC to the instrument.
2. Turn on the instrument.
3. Open the BindIt software and click the Connect button.
4. Select "Find New Instrument." BindIt will automatically connect to the instrument and the instrument screen will say "Computer Control".
5. Click the "Transfer" Button.
6. Click the "Upload" button.
7. Browse to find the file and double-click it.
8. In the new window that opens:
a. KingFisher Flex: you will be prompted to enter a new name – this is not necessary if the name only contains letters, digits, dash or underscore.
• If the name contains spaces, apostrophes or any other character, you should enter a new name containing only letters, digits, dash (-) or underscore (_).
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
b. KingFisher Duo/Duo Prime: The KingFisher Duo and Duo Prime instruments accept all characters in the filename.
• Click the "Category" pulldown to select the instrument folder you want to load the script to.
• Click OK.
Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.
The MagMAX Express-96 protocol scripts can be found on the MagMAX Express protocol page (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/rna-extraction/rna-types/total-rna-extraction/magmax-technology/magmax-particle-processors/magmax-instrument-protocols-scripts.html). If the specific script you are looking for is not located here or on the product page for the MagMAX kit, please contact Technical Support at techsupport@thermofisher.com.
We recommend using the BindIt Software to transfer scripts to the MagMAX Express-96 and KingFisher Flex instruments. The BindIt Software should be used to transfer scripts for all of the Applied Biosystems MagMAX Express and Thermo Scientific KingFisher instruments.
BindIt Software is used to operate the KingFisher 96, KingFisher mL, KingFisher Flex, KingFisher Duo Prime, and KingFisher Presto instruments. With the software you can:
• Create a plate layout
• Create new or modify existing protocols
• Transfer protocols to the KingFisher instrument memory
• Delete protocols from the KingFisher instrument
• Execute a protocol directly from the software without first sending it to the KingFisher instrument memory
• Export protocols to other databases
• Import protocols to the PC
• View protocol status reports
The end-user license agreement (EULA) for the BindIt Software can only be found in the install file. So you have to download the file, open it, then read the EULA, and finish installing the software.
There is no way to edit a BindiT protocol that has been installed on the KingFisher Flex/KingFisher DuoPrime instrument. The only way to edit the protocol is by saving the original .bdz file to a PC and then editing it in BindiT software.
The KingFisher Flex instrument needs to be connected to the computer/laptop and recognized by it. The lower bottom screen should say which instrument it is connected to. If it says anything about “simulator”, it is not connected to a real instrument.
You need to add a new instrument to the database in order to recognize the instrument: Settings > Instrument Setup > New > select what kind of instrument it is from the drop-down menu (i.e., KingFisher 96/MagMAX Express 96), give the instrument a name, and enter the serial number (make sure to keep the “-” in the middle). Once the instrument is connected and recognized, you should be able to transfer files over.
A .kf2 file cannot be edited in the BindIt software, but it is possible to rewrite a .kf2 file as a .bdz file so that it can be edited. Please see this application note for instructions on how to convert a .kf2 file into a .bdz file (http://www.thermoscientific.com/content/dam/tfs/LPG/LCD/LCD Documents/Application & Technical Notes/Liquid Handling and Dispensing/KingFisher-BindIT-TechNote-5189009.pdf). The speed settings are different in the two software (KingFisher software has 13 speed settings and BindIt software has 5 speed settings), so the application note has a conversion table for what speed to use in the BindIt software.
Yes, you can transfer a .kf2 file to a MagMAX Express-96 instrument using the BindIt software, but you need to have the correct USB to serial port converter.
The old KingFisher 2.6 software only works on Windows XP, whereas the BindIt software works on Windows XP, Windows 7, and Windows 8. The speed selections are also different. The old KingFisher 2.6 software featured 13 different speed setting whereas the BindIt software features 5 mixing speeds (slow, medium, fast, bottom, and half mix). The BindIt software can run KingFisher Software 2.6 files (.kf2) just as they are, but it cannot edit the .kf2 file. Please review our Technical Note for instructions on how to convert a .kf2 file to a format that can be edited in the BindIt software. A .kf2 protocol thas has been converted into a BindIt protocol can still achieve excellent yield and purity of the extracted target. Minor optimization may be required due to the differences in mix speeds and bead collection parameters, or to minimize protocol times.
Yes, the MagMAX Express-96 instrument is compatible with the BindIt software. A serial port-to-USB converter is required to connect the MagMAX Express-96 instrument to the computer.
If you are using BindIt software v3.3.1.7 you can use it to run existing .kf2 files. You will not be able to edit the .kf2 files in v3.3.1.7.
If you are using BindIt software v4.0 or later, you cannot run or edit existing .kf2 files. To get around this, you can convert the .kf2 files to the BindIt (.bdz) file format and then edit the .bdz file in the BindIt software. Please see the following application note for instructions on how to convert the .kf2 files: (http://www.thermoscientific.com/content/dam/tfs/LPG/LCD/LCD Documents/Application & Technical Notes/Liquid Handling and Dispensing/KingFisher-BindIT-TechNote-5189009.pdf). If you have already converted your .kf2 files to .bdz files, or you plan to convert your .kf2 file to .bdz files, we recommend using BindIt software v4.0 or higher.
BindIt software v3.3.1 is compatible with Windows XP (Professional with Service Pack 3), Windows Vista (Service Pack 2), Windows 7 (Service Pack 1), and Windows 8.
BindIT software v4 is compatible with Windows 7 with Service Pack 1 (32- or 64-bit), Windows 8.1 (32- or 64-bit), and Windows 10 (32- or 64-bit).