Amphiregulin causes functional downregulation of adherens junctions in psoriasis.
AuthorsChung E, Cook PW, Parkos CA, Park YK, Pittelkow MR, Coffey RJ,
JournalJ Invest Dermatol
PubMed ID15955087
'Overexpression of amphiregulin (AR) has been linked to psoriasis in mouse and man. Since psoriasis is marked by hyperproliferation of keratinocytes and loss of epidermal barrier function with infiltration of inflammatory cells into the epidermis and dermis, we hypothesized that AR might contribute to the pathogenesis of psoriasis by affecting ... More
Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.
AuthorsIvanov AI, Nusrat A, Parkos CA,
JournalMol Biol Cell
PubMed ID14528017
'The adherens junction (AJ) and tight junction (TJ) are key regulators of epithelial polarity and barrier function. Loss of epithelial phenotype is accompanied by endocytosis of AJs and TJs via unknown mechanisms. Using a model of calcium depletion, we defined the pathway of internalization of AJ and TJ proteins (E-cadherin, ... More
The tight junction protein ZO-2 localizes to the nucleus and interacts with the heterogeneous nuclear ribonucleoprotein scaffold attachment factor-B.
'Zonula occludens proteins (ZOPs), currently comprising ZO-1, ZO-2, and ZO-3, belong to the family of membrane-associated guanylate kinase homologue (MAGUK) proteins that are involved in the organization of epithelial and endothelial intercellular junctions. ZOPs bind to the cytoplasmic C termini of junctional transmembrane proteins linking them to the actin cytoskeleton. ... More
Claudin localization in cilia of the retinal pigment epithelium.
AuthorsNishiyama K, Sakaguchi H, Hu JG, Bok D, Hollyfield JG,
JournalAnat Rec
PubMed ID12115268
'Using immunocytochemistry and confocal microscopy we demonstrate that claudin-immunoreactivity is a novel marker for retinal pigment epithelial cilia. Claudin-immunoreactivity obtained by polyclonal anti-claudin 1 antibody, which could crossreact with claudin 3, was colocalized with acetylated tubulin-immunoreactivity in cultured human retinal pigment epithelial cells. Claudin-immunoreactivity associated with the retinal pigment epithelium ... More
Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.
'Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are ... More
Cell junctional proteins in the human corpus luteum: changes during the normal cycle and after HCG treatment.
'Regulation of tissue remodelling and ovarian permeability by intercellular adhesion complexes may be involved in normal and pathological ovarian function. Therefore, the occurrence, distribution and hormonal control of the adherens junction protein vascular endothelial cadherin (VE-cadherin) and the tight junction proteins occludin and claudin in the human corpus luteum (CL) ... More
The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex.
AuthorsMertens AE, Rygiel TP, Olivo C, van der Kammen R, Collard JG,
JournalJ Cell Biol
PubMed ID16186252
'The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) ... More
Distribution of tight junction proteins in adult human salivary glands.
AuthorsMaria OM, Kim JW, Gerstenhaber JA, Baum BJ, Tran SD
JournalJ Histochem Cytochem
PubMed ID18765838
'Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to ... More
Cyclooxygenase-2 deficiency leads to intestinal barrier dysfunction and increased mortality during polymicrobial sepsis.
AuthorsFredenburgh LE, Velandia MM, Ma J, Olszak T, Cernadas M, Englert JA, Chung SW, Liu X, Begay C, Padera RF, Blumberg RS, Walsh SR, Baron RM, Perrella MA
JournalJ Immunol
PubMed ID21967897
'Sepsis remains the leading cause of death in critically ill patients, despite modern advances in critical care. Intestinal barrier dysfunction may lead to secondary bacterial translocation and the development of the multiple organ dysfunction syndrome during sepsis. Cyclooxygenase (COX)-2 is highly upregulated in the intestine during sepsis, and we hypothesized ... More
Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells.
AuthorsYu AS, McCarthy KM, Francis SA, McCormack JM, Lai J, Rogers RA, Lynch RD, Schneeberger EE,
JournalAm J Physiol Cell Physiol
PubMed ID15689410
The function of occludin (Occ) in the tight junction is undefined. To gain insight into its role in epithelial cell biology, occludin levels in Madin-Darby canine kidney II cells were suppressed by stably expressing short interfering RNA. Suppression of occludin was associated with a decrease in claudins-1 and -7 and ... More
Differential expression of claudin tight junction proteins in the human cortical nephron.
AuthorsKirk A, Campbell S, Bass P, Mason J, Collins J
JournalNephrol Dial Transplant
PubMed ID20124215
In renal tubules, paracellular permeability is tightly controlled to facilitate solute absorption and urinary concentration and is regulated by tight junctions, which incorporate claudin proteins. There is very limited information confirming the localization of these proteins in the human renal cortex. Most data is inferred from mouse, bovine and rabbit ... More
Blood-testis barrier dynamics are regulated by testosterone and cytokines via their differential effects on the kinetics of protein endocytosis and recycling in Sertoli cells.
AuthorsYan HH, Mruk DD, Lee WM, Cheng CY,
JournalFASEB J
PubMed ID18192323
During spermatogenesis in the mammalian testis, preleptotene/leptotene spermatocytes differentiate from type B spermatogonia and traverse the blood-testis barrier (BTB) at stage VIII of the seminiferous epithelial cycle for further development. This timely movement of germ cells involves extensive junction restructuring at the BTB. Previous studies have shown that these events ... More
Immunocytochemical localization of claudin-1 in the maturation ameloblasts of rat incisors.
AuthorsNishikawa S, Abe M
JournalFront Physiol
PubMed ID21423390
Claudin-1 is a tight junction transmembrane protein. Its localization in the maturation ameloblasts of rat incisors was examined by immunofluorescence microscopy. Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive with anti-claudin-1 antibodies. Since it has been ... More
Inducible expression of Snail selectively increases paracellular ion permeability and differentially modulates tight junction proteins.
AuthorsCarrozzino F, Soulié P, Huber D, Mensi N, Orci L, Cano A, Féraille E, Montesano R,
JournalAm J Physiol Cell Physiol
PubMed ID15930145
Constitutive expression of the transcription factor Snail was previously shown to trigger complete epithelial-mesenchymal transition (EMT). The aim of this study was to determine whether inducible expression of Snail could modify epithelial properties without eliciting full mesenchymal conversion. For this purpose, we expressed mouse Snail (mSnail) cDNA in Madin-Darby canine ... More
Identification of claudins by western blot and immunofluorescence in different cell lines and tissues.
AuthorsGonzález-Mariscal L, Garay E, Quirós M,
JournalMethods Mol Biol
PubMed ID21717359
Claudins are integral proteins of the TJ. Each epithelia in the organism expresses a unique set of claudins that determines the degree of sealing of the paracellular pathway and the ionic selectivity of the tissue. TJs are dynamic structures whose organization and composition change in response to alterations in the ... More
CRTAM: A molecule involved in epithelial cell adhesion.
AuthorsGaray E, Patiño-López G, Islas S, Alarcón L, Canche-Pool E, Valle-Rios R, Medina-Contreras O, Granados G, Chávez-Munguía B, Juaristi E, Ortiz-Navarrete V, González-Mariscal L,
JournalJ Cell Biochem
PubMed ID20556794
Class I-restricted T cell associated molecule (CRTAM) is a member of the immunoglobulin superfamily that complies with the structural characteristics of the JAM family of proteins and is phylogenetically more closely related to nectin-like proteins. Here we demonstrate for the first time, that CRTAM is expressed in epithelial cells along ... More
Prostate cancer progression into androgen independency is associated with alterations in cell adhesion and invasivity.
AuthorsJennbacken K, Gustavsson H, Welén K, Vallbo C, Damber JE,
JournalProstate
PubMed ID16927303
Mortality in prostate cancer is primarily due to failure to cure hormone refractory patients with metastatic disease. The present study focused on elucidating alterations in invasive properties, which are connected with progression into androgen independency. Ability to grow without anchor, migration, cell adhesion properties and expression of invasive factors were ... More
erbB1 functions as a sensor of airway epithelial integrity by regulation of protein phosphatase 2A activity.
AuthorsVermeer PD, Panko L, Welsh MJ, Zabner J,
JournalJ Biol Chem
PubMed ID16293617
Two enzymes, protein phosphatase 2A and atypical protein kinase C, are associated with the tight junction and regulate its function. For example, phosphorylation of the tight junction protein occludin is required for its incorporation into the junction. The association of a kinase and phosphatase with the tight junction suggests that ... More
Changes in the distribution of ZO-1, occludin, and claudins in the rat uterine epithelium during the estrous cycle.
AuthorsMendoza-Rodríguez CA, González-Mariscal L, Cerbón M,
JournalCell Tissue Res
PubMed ID15558325
During the estrous cycle, the endometrium epithelium experiences marked cellular structural changes. For fertilization to proceed, maintenance of an adequate uterine environment by ovarian hormones is essential. Epithelial cells lining the uterine lumen are associated with each other by tight junctions (TJs), which regulate the passage of ions and molecules ... More
Scribble regulates an EMT polarity pathway through modulation of MAPK-ERK signaling to mediate junction formation.
AuthorsElsum IA, Martin C, Humbert PO,
Journal
PubMed ID23813956
The crucial role the Crumbs and Par polarity complexes play in tight junction integrity has long been established, however very few studies have investigated the role of the Scribble polarity module. Here, we use MCF10A cells, which fail to form tight junctions and express very little endogenous Crumbs3, to show ... More
Physiology of blood-brain interfaces in relation to brain disposition of small compounds and macromolecules.
AuthorsStrazielle N, Ghersi-Egea JF,
JournalMol Pharm
PubMed ID23298398
The brain develops and functions within a strictly controlled environment resulting from the coordinated action of different cellular interfaces located between the blood and the extracellular fluids of the brain, which include the interstitial fluid and the cerebrospinal fluid (CSF). As a correlate, the delivery of pharmacologically active molecules and ... More
Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells.
AuthorsIkari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J,
Journal
PubMed ID24659781
Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg(2+) in the thick ascending limb (TAL) of Henle's loop. However, the mechanism regulating the tight junctional localization of CLDN16 remains unknown. In yeast two-hybrid systems, we found that CLDN16 bound to syntaxin 8 (STX8), a target soluble N-ethylmaleimide-sensitive factor attachment protein receptor. ... More
Functional organization of the bovine rumen epithelium.
AuthorsGraham C, Simmons NL,
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID15319221
The functional organization of the bovine rumen epithelium has been examined by electron and light microscopy combined with immunocytochemistry to define a transport model for this epithelium. Expression of connexin 43, an integral component of gap junctions, the tight-junction molecules claudin-1 and zonula occludens 1 (ZO-1), and the catalytic alpha-subunit ... More
Isoforms of the polarity protein par6 have distinct functions.
AuthorsGao L, Macara IG,
JournalJ Biol Chem
PubMed ID15292221
PAR-6 is essential for asymmetric division of the Caenorhabditis elegans zygote. It is also critical for cell polarization in many other contexts throughout the Metazoa. The Par6 protein contains a PDZ domain and a partial CRIB (Cdc42/Rac interactive binding) domain, which mediate interactions with other polarity proteins such as Par3, ... More
Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells.
AuthorsAmasheh S, Meiri N, Gitter AH, Schöneberg T, Mankertz J, Schulzke JD, Fromm M,
JournalJ Cell Sci
PubMed ID12432083
Tight junctions seal the paracellular pathway of epithelia but, in leaky tissues, also exhibit specific permeability. In order to characterize the contribution of claudin-2 to barrier and permeability properties of the tight junction in detail, we studied two strains of Madin-Darby canine kidney cells (MDCK-C7 and MDCK-C11) with different tight ... More
Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms.
AuthorsMcLaughlin J, Padfield PJ, Burt JP, O'Neill CA,
JournalAm J Physiol Cell Physiol
PubMed ID15229101
On interaction with the intestine, the mycotoxin ochratoxin A is know to cause rapid inflammation, diarrhea, and increased bacterial translocation. All these effects are consistent with a decrease in epithelial barrier function. However, this has not been shown directly. We determined that ochratoxin A is able to reduce the barrier ... More
Bryostatin-1 enhances barrier function in T84 epithelia through PKC-dependent regulation of tight junction proteins.
AuthorsYoo J, Nichols A, Mammen J, Calvo I, Song JC, Worrell RT, Matlin K, Matthews JB,
JournalAm J Physiol Cell Physiol
PubMed ID12660149
Protein kinase C (PKC) is known to regulate epithelial barrier function. However, the effect of specific PKC isozymes, and their mechanism of action, are largely unknown. We determined that the nonphorbol ester PKC agonist bryostatin-1 increased transepithelial electrical resistance (TER), a marker of barrier function, in confluent T84 epithelia. Bryostatin-1, ... More
Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture.
AuthorsColegio OR, Van Itallie C, Rahner C, Anderson JM,
JournalAm J Physiol Cell Physiol
PubMed ID12700140
Tight junctions (TJs) regulate paracellular permeability across epithelia and vary widely in their transepithelial electrical resistance (TER) and charge selectivity. The claudin family of transmembrane proteins influences these properties. We previously reported that claudin-4 increased TER approximately 300% when expressed in low-resistance Madin-Darby canine kidney (MDCK) II cells and decreased ... More
Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.
AuthorsKratzer I, Vasiljevic A, Rey C, Fevre-Montange M, Saunders N, Strazielle N, Ghersi-Egea JF,
JournalHistochem Cell Biol
PubMed ID22886143
The choroid plexus epithelium controls the movement of solutes between the blood and the cerebrospinal fluid. It has been considered as a functionally more immature interface during brain development than in adult. The anatomical basis of this barrier is the interepithelial choroidal junction whose tightness has been attributed to the ... More
Effects of proinflammatory cytokines on the claudin-19 rich tight junctions of human retinal pigment epithelium.
AuthorsPeng S, Gan G, Rao VS, Adelman RA, Rizzolo LJ,
JournalInvest Ophthalmol Vis Sci
PubMed ID22761260
Chronic, subclinical inflammation contributes to the pathogenesis of several ocular diseases, including age-related macular degeneration. Proinflammatory cytokines affect tight junctions in epithelia that lack claudin-19, but in the retinal pigment epithelium claudin-19 predominates. We examined the effects of cytokines on the tight junctions of human fetal RPE (hfRPE). hfRPE was ... More
Paracellular Cl- permeability is regulated by WNK4 kinase: insight into normal physiology and hypertension.
AuthorsKahle KT, Macgregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP,
JournalProc Natl Acad Sci U S A
PubMed ID15465913
Paracellular ion flux across epithelia occurs through selective and regulated pores in tight junctions; this process is poorly understood. Mutations in the kinase WNK4 cause pseudohypoaldosteronism type II (PHAII), a disease featuring hypertension and hyperkalemia. Whereas WNK4 is known to regulate several transcellular transporters and channels involved in NaCl and ... More
Cultured monolayers of the dog jejunum with the structural and functional properties resembling the normal epithelium.
AuthorsWeng XH, Beyenbach KW, Quaroni A,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15550553
The development of a culture of the normal mammalian jejunum motivated this work. Isolated crypt cells of the dog jejunum were induced to form primary cultures on Snapwell filters. Up to seven subcultures were studied under the electron microscope and in Ussing chambers. Epithelial markers were identified by RT-PCR, Western ... More
Transmigration of neural stem cells across the blood brain barrier induced by glioma cells.
AuthorsDíaz-Coránguez M, Segovia J, López-Ornelas A, Puerta-Guardo H, Ludert J, Chávez B, Meraz-Cruz N, González-Mariscal L,
JournalPLoS One
PubMed ID23637756
Transit of human neural stem cells, ReNcell CX, through the blood brain barrier (BBB) was evaluated in an in vitro model of BBB and in nude mice. The BBB model was based on rat brain microvascular endothelial cells (RBMECs) cultured on Millicell inserts bathed from the basolateral side with conditioned ... More
Drebrin, an actin-binding, cell-type characteristic protein: induction and localization in epithelial skin tumors and cultured keratinocytes.
AuthorsPeitsch WK, Hofmann I, Bulkescher J, Hergt M, Spring H, Bleyl U, Goerdt S, Franke WW
JournalJ Invest Dermatol
PubMed ID16185277
Isoform E2 of drebrin, an actin-binding protein originally identified in neuronal cells, has recently been identified in diverse non-neuronal cells, mostly in association with cell processes and intercellular junctions. Here, we report on the presence of drebrin in normal human skin, epithelial skin cancers, and cultured keratinocytes. Keratinocytes of normal ... More
Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells.
AuthorsPoliak S, Matlis S, Ullmer C, Scherer SS, Peles E
JournalJ Cell Biol
PubMed ID12403818
The apposed membranes of myelinating Schwann cells are joined by several types of junctional specializations known as autotypic or reflexive junctions. These include tight, gap, and adherens junctions, all of which are found in regions of noncompact myelin: the paranodal loops, incisures of Schmidt-Lanterman, and mesaxons. The molecular components of ... More
Functional ESCRT machinery is required for constitutive recycling of claudin-1 and maintenance of polarity in vertebrate epithelial cells.
AuthorsDukes JD, Fish L, Richardson JD, Blaikley E, Burns S, Caunt CJ, Chalmers AD, Whitley P
JournalMol Biol Cell
PubMed ID21757541
Genetic screens in Drosophila have identified regulators of endocytic trafficking as neoplastic tumor suppressor genes. For example, Drosophila endosomal sorting complex required for transport (ESCRT) mutants lose epithelial polarity and show increased cell proliferation, suggesting that ESCRT proteins could function as tumor suppressors. In this study, we show for the ... More