A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.
AuthorsAlawar N,Schirra C,Hohmann M,Becherer U
JournalBMC biotechnology
PubMed ID38532411
BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and ... More