UltraLink™ 酰肼树脂
UltraLink™ 酰肼树脂
Thermo Scientific™

UltraLink™ 酰肼树脂

Thermo Scientific UltraLink 酰肼树脂用于制备含抗体或其他糖基化蛋白的亲和柱。UltraLink 酰肼树脂的特点:•高容量—可固定 1 至 10 mg了解更多信息
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货号数量
5314910 mL
货号 53149
价格(CNY)
4,363.00
Each
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数量:
10 mL
请求批量或定制报价
价格(CNY)
4,363.00
Each
添加至购物车
Thermo Scientific UltraLink 酰肼树脂用于制备含抗体或其他糖基化蛋白的亲和柱。

UltraLink 酰肼树脂的特点:

高容量—可固定 1 至 10 mg 氧化抗体或其他糖蛋白/毫升树脂
快速、高效—在少至 30 分钟内固定;在不超过 4 小时内实现至少 90% 的大多数糖蛋白偶联(效率取决于糖基化的量和类型)
特异性固定—酰肼活化 UltraLink 树脂仅与糖(如唾液酸)基团经过高碘酸盐温和氧化的纯化糖蛋白偶联
稳定连接—在使用一个制备树脂批次进行的多种亲和纯化中,腙键的共振结构足够稳定;无需稳定步骤
保留抗体功能—通过 Fc 区固定 IgG,从而保留用于捕获靶标的两个抗原结合位点

酰肼活化树脂用于制备含多克隆抗体的亲和柱,这些抗体通常在 Fc 部分具有高丰度碳水化合物(糖基化)。任何含高碘酸盐-可氧化碳水化合物基团的糖基化蛋白(甚至单克隆抗体)都可固定化以制备可重复使用的亲和柱。采用酰肼固定化学技术固定的抗体具有无遮挡的抗原结合位点和极佳的纯化能力。用稳定糖蛋白或抗体制备的柱在亲和纯化中可再生并重复使用至少 5 次,不会在结合能力方面造成重大损失。

应用:
•使用共价偶联抗体纯化抗原
• 确定目标糖蛋白的结合配偶体
• 进行大规模亲和纯化

UltraLink 酰肼树脂对于糖蛋白(如多克隆抗体)特别有用,因为其可以使分子连接在不干扰活性结合位点的结构域,而这些位点对预期的亲和纯化至关重要。糖蛋白的碳水化合物部分包含常见的带顺式二醇基团(易于通过偏高碘酸钠氧化以产生醛)的糖。当与酰肼树脂一起孵育时,这些醛基与树脂上的酰肼基反应,可形成稳定的共价键。Thermo Scientific GlycoLink 耦合催化剂可提高偶联效率并缩短孵育时间,从而增强该反应。整个偶联反应发生于含催化剂的简单非胺缓冲液中(2 至 4 小时)。与抗体和典型糖蛋白的偶联效率通常高于 85%,得到 1 至 10 mg 固定蛋白/mL Thermo Scientific UltraLink 酰肼树脂。

更多产品数据
改善糖蛋白固定和偶联

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GlycoLink™ 固定试剂盒
GlycoLink™ 微固定试剂盒
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和,UltraLink™ 树脂
描述UltraLink 酰肼树脂
产品规格瓶装
数量10 mL
固定相酰肼
容量(公制)固定 1-10 mg 氧化抗体或糖蛋白/mL 树脂
产品线UltraLink™
类型树脂
Unit SizeEach
内容与储存
储存于 4°C。

常见问题解答 (FAQ)

How many times can the same affinity column in the GlycoLink Immobilization Kit be used for purification?

The stability of the immobilized protein and the type of elution buffer used determines how many times a column can be reused. Typically, the columns can be reused at least five times without significant loss in performance.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Will Hydrazide UltraLink Resins in the GlycoLink Immobilization kit couple chicken IgY?

Yes. Chicken antibodies have a total of 4 N-linked glycosylation sites and will couple to the resin.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can the Hydrazide UltraLink Resin in the GlycoLink Immobilization kit couple all types of antibodies?

The resin will couple any antibody that is adequately glycosylated. Although monoclonal antibodies are typically under-glycosylated, many have been effectively immobilized to this resin. To determine if a monoclonal antibody is glycosylated use the Thermo Scientific Glycoprotein Carbohydrate Estimation Kit (Cat. No. 23260).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

When oxidizing with sodium meta-periodate, is it possible to over-oxidize the antibody when using the GlycoLink Immobilization Kit?

If too much sodium meta-periodate is used or the sample is incubated for too long the oxidation can cause the protein to breakdown or favor an amine linkage to the resin. Furthermore, if used in excess, methionine residues might become oxidized. Typically, using 10 mM sodium meta-periodate for 30 minutes will oxidize only the carbohydrates and does not affect protein function.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How do I oxidize a glycoprotein or antibody when using the GlycoLink Immobilization Kit?

The antibody or other glycoprotein is first diluted or desalted into the coupling buffer and transferred to a vial containing sodium meta-periodate at a final concentration of 10 mM (1 mM is sufficient for sialic acid residues). The mixture is incubated for 30 minutes at room temperature in the dark. Sodium meta-periodate concentration can be increased to 25mM if oxidation is not sufficient at 10 mM. Care must be taken not to over-oxidize the glycoprotein.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.