SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
Thermo Scientific™

SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis

Thermo Scientific™ SMART Digest™ Dual Enzyme Columns for HDX-MS analysis provide fast, reliable digestion of proteins for HDX-MS analysis, helping to provide the sequence characterization of biotherapeutics.

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货号 60160-110
价格（CNY)
21,868.24
Each
添加至购物车
价格（CNY)
21,868.24
Each
添加至购物车

Thermo Scientific SMART Digest Dual Enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.

SMART Digest Dual Enzyme columns for HDX-MS analysis help to provide the characterization of biotherapeutics.

The combination of enzymes are complementary, creating many short, overlapping N-terminal peptides for a high degree of sequence coverage.

Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. The optimum pH range for pepsin activity is 1.0–3.0.

Proteinase XIII cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.

规格
色谱柱格式Immobilized enzyme
色谱柱类型Immobilized enzyme in PEEK cartridge
描述SMART Digest Pepsin/Protease XIII Dual Enzyme Column
直径2.1 mm
流速10 to 500 μL/min
适用于（应用）HDX-MS analysis
长度30 mm
最大压力2500 psi (170 bar)
流动相兼容性Phosphate buffer pH 4
工作温度范围4 °C to 50 °C
包装材料Hydrophilic/coated DVB
粒径˜20 μm
孔径° 1000 angstroms
稳定性1 year
固定相Immobilized enzyme
储存温度RT for up to 2 weeks; 4 °C for extended period (refrigerate in MES pH 6)
技术BioLC
重量8 g
切割Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine. The optimum pH range for pepsin activity is 1.0–3.0.

Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine). Like pepsin, Proteinase XIII is active at low pH.
é…¶Pepsin & Protease XIII
类型SMART Digest Pepsin/Protease XIII Dual Enzyme column
pH2 to 6
Unit SizeEach