Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo dT microspheres.
AuthorsJakobsen KS, Breivold E, Hornes E
JournalNucleic Acids Res
PubMed ID2362831
Induction of nitric oxide synthase mRNA in coronary resistance arteries isolated from exercise-trained pigs.
AuthorsWoodman CR, Muller JM, Laughlin MH, Price EM
JournalAm J Physiol
PubMed ID9435589
The purpose of this study was to develop a method by which endothelial cell nitric oxide synthase (ecNOS) mRNA expression could be measured in single coronary resistance arteries and to test the hypothesis that ecNOS gene expression is upregulated by exercise training. Yucatan miniature swine were randomly assigned to exercise-trained ... More
Disruption of imprinted gene methylation and expression in cloned preimplantation stage mouse embryos.
Cloning by somatic cell nuclear transfer requires that epigenetic information possessed by the donor nucleus be reprogrammed to an embryonic state. Little is known, however, about this remodeling process, including when it occurs, its efficiency, and how well epigenetic markings characteristic of normal development are maintained. Examining the fate of ... More
Selective loss of imprinting in the placenta following preimplantation development in culture.
AuthorsMann MR, Lee SS, Doherty AS, Verona RI, Nolen LD, Schultz RM, Bartolomei MS
JournalDevelopment
PubMed ID15240554
Preimplantation development is a period of dynamic epigenetic change that begins with remodeling of egg and sperm genomes, and ends with implantation. During this time, parental-specific imprinting marks are maintained to direct appropriate imprinted gene expression. We previously demonstrated that H19 imprinting could be lost during preimplantation development under certain ... More
In vitro characterization of somatostatin receptors in the human thymus and effects of somatostatin and octreotide on cultured thymic epithelial cells.
AuthorsFerone D, van Hagen PM, van Koetsveld PM, Zuijderwijk J, Mooy DM, Lichtenauer-Kaligis EG, Colao A, Bogers AJ, Lombardi G, Lamberts SW, Hofland LJ
JournalEndocrinology
PubMed ID9886848
Somatostatin (SS) and its analogs exert inhibitory effects on secretive and proliferative processes of various cells via high affinity SS receptors (SS-R). SS analogs bind with different affinity to the five cloned SS-R subtypes. Octreotide, an octapeptide SS analog, binds with high affinity to the SS-R subtype 2 (sst2). SS-R ... More