Epoxyeicosatrienoic acids and their sulfonimide derivatives stimulate tyrosine phosphorylation and induce mitogenesis in renal epithelial cells.
AuthorsChen JK, Falck JR, Reddy KM, Capdevila J, Harris RC
JournalJ Biol Chem
PubMed ID9786938
'In our present studies utilizing a well characterized proximal tubule cell line, LLCPKcl4, we determined that all four EET regioisomers (5,6-EET, 8,9-EET, 11,12-EET, and 14,15-EET) stimulated [3H]thymidine incorporation, with 14,15-EET being the most potent. In contrast, no mitogenic effects were seen with arachidonic acid, other cP450 arachidonate metabolites (12R-hydroxyeicosatetraenoic acid ... More
Changes in the mitochondrial phosphoproteome during mammalian hibernation.
AuthorsChung DJ, Szyszka B, Brown JC, Hüner NP, Staples JF,
JournalPhysiol Genomics
PubMed ID23572536
'Mammalian hibernation involves periods of substantial suppression of metabolic rate (torpor) allowing energy conservation during winter. In thirteen-lined ground squirrels (Ictidomys tridecemlineatus), suppression of liver mitochondrial respiration during entrance into torpor occurs rapidly (within 2 h) before core body temperature falls below 30°C, whereas reversal of this suppression occurs slowly ... More
D1 dopamine receptor regulation of microtubule-associated protein-2 phosphorylation in developing cerebral cortical neurons.
This study addresses the hypothesis that the previously described capacity of D1 dopamine receptors (D1Rs) to regulate dendritic growth in developing cortical neurons may involve alterations in the phosphorylation state of microtubule-associated protein-2 (MAP2). The changes in phosphorylation of this protein are known to affect its ability to stabilize the ... More
Phospholipase C-gamma1 is required for the induction of immediate early genes by platelet-derived growth factor.
AuthorsLiao HJ, Ji QS, Carpenter G,
JournalJ Biol Chem
PubMed ID11254653
To explore the functional role of phospholipase C-gamma1 (PLC-gamma1) in the induction of immediate early genes (IEGs), we have examined the influence of Plcg1 gene disruption on the expression of 14 IEG mRNAs induced by platelet-derived growth factor (PDGF). Plcg1-null embryos were used to produce immortalized fibroblasts genetically deficient in ... More
Evidence for an essential catalytic role of the F10 protein kinase in vaccinia virus morphogenesis.
AuthorsSzajner P, Weisberg AS, Moss B,
JournalJ Virol
PubMed ID14671107
Temperature-sensitive mutants of vaccinia virus, with genetic changes that map to the open reading frame encoding the F10 protein kinase, exhibit a defect at an early stage of viral morphogenesis. To further study the role of the enzyme, we constructed recombinant vaccinia virus vF10V5i, which expresses inducible V5 epitope-tagged F10 ... More
v-Crk activates the phosphoinositide 3-kinase/AKT pathway in transformation.
AuthorsAkagi T, Shishido T, Murata K, Hanafusa H,
JournalProc Natl Acad Sci U S A
PubMed ID10852971
v-Crk induces cellular tyrosine phosphorylation and transformation of chicken embryo fibroblasts (CEF). We studied the molecular mechanism of the v-Crk-induced transformation. Experiments with Src homology (SH)2 and SH3 domain mutants revealed that the induction of tyrosine phosphorylation of cellular proteins requires only the SH2 domain, but both the SH2 and ... More
Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor.
AuthorsSu L, Mruk DD, Cheng CY,
JournalAm J Physiol Cell Physiol
PubMed ID22875787
The blood-testis barrier (BTB) divides the seminiferous epithelium into the basal and the adluminal compartment. It restricts paracellular diffusion of molecules between Sertoli cells, confers cell polarity, and creates a unique microenvironment in the adluminal compartment for spermatid development. However, it undergoes restructuring during the epithelial cycle so that preleptotene ... More
Transfection of an active cytochrome P450 arachidonic acid epoxygenase indicates that 14,15-epoxyeicosatrienoic acid functions as an intracellular second messenger in response to epidermal growth factor.
AuthorsChen JK, Wang DW, Falck JR, Capdevila J, Harris RC
JournalJ Biol Chem
PubMed ID9988714
A common feature of most isolated cell systems is low or undetectable levels of bioactive cytochrome P450. We therefore developed stable transfectants of the renal epithelial cell line, LLCPKcl4, that expressed an active regio- and enantioselective arachidonic acid (AA) epoxygenase. Site-specific mutagenesis was used to convert bacterial P450 BM-3 into ... More