Focal adhesion kinase-Tyr407 and -Tyr397 exhibit antagonistic effects on blood-testis barrier dynamics in the rat.
AuthorsLie PP, Mruk DD, Mok KW, Su L, Lee WM, Cheng CY,
JournalProc Natl Acad Sci U S A
PubMed ID22797892
'Focal adhesion kinase (FAK), a nonreceptor protein tyrosine kinase, displays phosphorylation-dependent localization in the seminiferous epithelium of adult rat testes. FAK is an integrated component of the blood-testis barrier (BTB) involved in regulating Sertoli cell adhesion via its effects on the occludin-zonula occludens-1 complex. Herein, we report that p-FAK-Tyr(407) and ... More
Gap junction adhesion is necessary for radial migration in the neocortex.
AuthorsElias LA, Wang DD, Kriegstein AR,
JournalNature
PubMed ID17713529
'Radial glia, the neuronal stem cells of the embryonic cerebral cortex, reside deep within the developing brain and extend radial fibres to the pial surface, along which embryonic neurons migrate to reach the cortical plate. Here we show that the gap junction subunits connexin 26 (Cx26) and connexin 43 (Cx43) ... More
Connexin 43 interacts with zona occludens-1 and -2 proteins in a cell cycle stage-specific manner.
AuthorsSingh D, Solan JL, Taffet SM, Javier R, Lampe PD
JournalJ Biol Chem
PubMed ID15980428
'Gap junction channels play an important role in cell growth control, secretion and embryonic development. Gap junctional communication and channel assembly can be regulated by protein-protein interaction with kinases and phosphatases. We have utilized tandem mass spectrometry (MS/MS) sequence analysis as a screen to identify proteins from cell lysates that ... More
Toward the generation of rod and cone photoreceptors from mouse, monkey and human embryonic stem cells.
AuthorsOsakada F, Ikeda H, Mandai M, Wataya T, Watanabe K, Yoshimura N, Akaike A, Akaike A, Sasai Y, Takahashi M,
JournalNat Biotechnol
PubMed ID18246062
'We previously reported the differentiation of mouse embryonic stem (ES) cells into retinal progenitors. However, these progenitors rarely differentiate into photoreceptors unless they are cultured with embryonic retinal tissues. Here we show the in vitro generation of putative rod and cone photoreceptors from mouse, monkey and human ES cells by ... More
Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.
AuthorsDamiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E,
JournalJ Neurosci
PubMed ID18463241
'MicroRNAs (miRNAs) are small, highly conserved molecules that have been shown to regulate the expression of genes by binding to specific target mRNAs. Dicer, an RNase III endonuclease, is essential for the production and function of mature miRNAs, and removal of Dicer has been shown to disrupt many developmental processes. ... More
Expression of kinase-inactive c-Src delays oxidative stress-induced disassembly and accelerates calcium-mediated reassembly of tight junctions in the Caco-2 cell monolayer.
'The activity of Src kinases appears to play a role in both assembly and disassembly of tight junction. However, the role of a specific isoform of Src kinase in regulation of tight junction is not known. In the present study the role of c-Src in regulation of epithelial tight junction ... More
Mast cell tryptase controls paracellular permeability of the intestine. Role of protease-activated receptor 2 and beta-arrestins.
'Tight junctions between intestinal epithelial cells prevent ingress of luminal macromolecules and bacteria and protect against inflammation and infection. During stress and inflammation, mast cells mediate increased mucosal permeability by unknown mechanisms. We hypothesized that mast cell tryptase cleaves protease-activated receptor 2 (PAR2) on colonocytes to increase paracellular permeability. Colonocytes ... More
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
AuthorsGovindarajan R, Zhao S, Song XH, Guo RJ, Wheelock M, Johnson KR, Mehta PP
JournalJ Biol Chem
PubMed ID12205082
'Gap junctions, composed of connexins, provide a pathway of direct intercellular communication for the diffusion of small molecules between cells. Evidence suggests that connexins act as tumor suppressors. We showed previously that expression of connexin-43 and connexin-32 in an indolent prostate cancer cell line, LNCaP, resulted in gap junction formation ... More
The short apical membrane half-life of rescued {Delta}F508-cystic fibrosis transmembrane conductance regulator (CFTR) results from accelerated endocytosis of {Delta}F508-CFTR in polarized human airway epithelial cells.
AuthorsSwiatecka-Urban A, Brown A, Moreau-Marquis S, Renuka J, Coutermarsh B, Barnaby R, Karlson KH, Flotte TR, Fukuda M, Langford GM, Stanton BA,
JournalJ Biol Chem
PubMed ID16131493
'The most common mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene in individuals with cystic fibrosis, DeltaF508, causes retention of DeltaF508-CFTR in the endoplasmic reticulum and leads to the absence of CFTR Cl(-) channels in the apical plasma membrane. Rescue of DeltaF508-CFTR by reduced temperature or chemical means ... More
Function of atypical protein kinase C lambda in differentiating photoreceptors is required for proper lamination of mouse retina.
'The photoreceptor is a highly polarized neuron and also has epithelial characteristics such as adherens junctions. To investigate the mechanisms of polarity formation of the photoreceptor cells, we conditionally knocked out atypical protein kinase Clambda (aPKClambda), which has been proposed to play a critical role in the establishment of epithelial ... More
Amphiregulin causes functional downregulation of adherens junctions in psoriasis.
AuthorsChung E, Cook PW, Parkos CA, Park YK, Pittelkow MR, Coffey RJ,
JournalJ Invest Dermatol
PubMed ID15955087
'Overexpression of amphiregulin (AR) has been linked to psoriasis in mouse and man. Since psoriasis is marked by hyperproliferation of keratinocytes and loss of epidermal barrier function with infiltration of inflammatory cells into the epidermis and dermis, we hypothesized that AR might contribute to the pathogenesis of psoriasis by affecting ... More
Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment.
AuthorsIvanov AI, Nusrat A, Parkos CA,
JournalMol Biol Cell
PubMed ID14528017
'The adherens junction (AJ) and tight junction (TJ) are key regulators of epithelial polarity and barrier function. Loss of epithelial phenotype is accompanied by endocytosis of AJs and TJs via unknown mechanisms. Using a model of calcium depletion, we defined the pathway of internalization of AJ and TJ proteins (E-cadherin, ... More
Polyamines are necessary for synthesis and stability of occludin protein in intestinal epithelial cells.
AuthorsGuo X, Rao JN, Liu L, Zou T, Keledjian KM, Boneva D, Marasa BS, Wang JY,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID15691870
'Occludin is an integral membrane protein that forms the sealing element of tight junctions and is critical for epithelial barrier function. Polyamines are implicated in multiple signaling pathways driving different biological functions of intestinal epithelial cells (IEC). The present study determined whether polyamines are involved in expression of occludin and ... More
rpS6 regulates blood-testis barrier dynamics through Akt-mediated effects on MMP-9.
AuthorsMok KW, Mruk DD, Cheng CY,
Journal
PubMed ID25217631
'Mammalian target of rapamycin complex 1 (mTORC1) is an emerging regulator of blood-tissue barriers that utilizes ribosomal protein S6 (rpS6) as the downstream signaling molecule. To explore the role of rpS6 in blood-testis barrier (BTB) function, a constitutively active quadruple rpS6 phosphomimetic mutant was constructed in mammalian expression vector and ... More
Neuronal connexin36 association with zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO-1.
AuthorsLi X, Olson C, Lu S, Kamasawa N, Yasumura T, Rash JE, Nagy JI,
JournalEur J Neurosci
PubMed ID15090040
'Among the 20 members in the connexin family of gap junction proteins, only connexin36 (Cx36) is firmly established to be expressed in neurons and to form electrical synapses at widely distributed interneuronal gap junctions in mammalian brain. Several connexins have recently been reported to interact with the PDZ domain-containing protein ... More
sPAR-3, a splicing variant of PAR-3, shows cellular localization and an expression pattern different from that of PAR-3 during enterocyte polarization.
'PAR-3 (partitioning-defective) is a scaffold-like PDZ (postsynaptic density-95/discs large/zonula occludens-1) domain-containing protein that forms a complex with PAR-6 and atypical PKC, localizes to tight junctions, and contributes to the formation of functional tight junctions. There are several alternatively spliced isoforms of PAR-3, although their physiological significance remains unknown. In this ... More
Rare incorporation of bone marrow-derived cells into kidney after folic acid-induced injury.
'Results obtained in recent experiments suggest that bone marrow-derived cells (BMDCs) engraft into tissues and differentiate into various somatic cell types. However, it is unclear whether injury is required for the phenomenon to occur at appreciable frequencies. In this study we tested whether BMDCs engraft into kidneys and differentiate into ... More
Protection against hypoxia-induced increase in blood-brain barrier permeability: role of tight junction proteins and NFkappaB.
AuthorsBrown RC, Mark KS, Egleton RD, Huber JD, Burroughs AR, Davis TP,
JournalJ Cell Sci
PubMed ID12538770
'Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight junction protein expression, and studied potential mechanisms of action. ... More
Truncation mutants of the tight junction protein ZO-1 disrupt corneal epithelial cell morphology.
AuthorsRyeom SW, Paul D, Goodenough DA,
JournalMol Biol Cell
PubMed ID10793144
'The tight junction is the most apical intercellular junction of epithelial cells and regulates transepithelial permeability through the paracellular pathway. To examine possible functions for the tight junction-associated protein ZO-1, C-terminally truncated mutants and a deletion mutant of ZO-1 were epitope tagged and stably expressed in corneal epithelial cell lines. ... More
The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction.
AuthorsNusrat A, Chen JA, Foley CS, Liang TW, Tom J, Cromwell M, Quan C, Mrsny RJ,
JournalJ Biol Chem
PubMed ID10887180
'Occludin is an integral membrane protein that has been suggested to play a role in the organization and dynamic function of the epithelial tight junction (TJ). A number of other proteins have also been described to localize to the TJ. We have used a novel bait peptide method to investigate ... More
Characterization of epithelial cell shedding from human small intestine.
'Intestinal epithelial cells migrate from the base of the crypt to the villi where they are shed. However, little is known about the cell shedding process. We have studied the role of apoptosis and wound healing mechanisms in cell shedding from human small intestinal epithelium. A method preparing paraffin sections ... More
Ablation of Cx47 in transgenic mice leads to the loss of MUPP1, ZONAB and multiple connexins at oligodendrocyte-astrocyte gap junctions.
AuthorsLi X, Penes M, Odermatt B, Willecke K, Nagy JI,
JournalEur J Neurosci
PubMed ID18973575
'Oligodendrocytes in CNS are linked to astrocytes by heterotypic gap junctions composed of Cx32 and Cx47 in oligodendrocytes and Cx30 and Cx43 in astrocytes. These gap junctions also harbour regulatory proteins, including ZO-1 and ZONAB. Here, we investigated the localization of multi-PDZ domain protein 1 (MUPP1) at these gap junctions ... More
Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells.
AuthorsChang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, Fukamachi H, Ito Y,
JournalGastroenterology
PubMed ID19706291
'The transcription factor RUNX3 is a gastric tumor suppressor. Tumorigenic Runx3(-/-) gastric epithelial cells attach weakly to each other, compared with nontumorigenic Runx3(+/+) cells. We aimed to identify RUNX3 target genes that promote cell-cell contact to improve our understanding of RUNX3''s role in suppressing gastric carcinogenesis. We compared gene expression ... More
Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.
'Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are ... More
Griffonia simplicifolia isolectin B4 identifies a specific subpopulation of angiogenic blood vessels following contusive spinal cord injury in the adult mouse.
AuthorsBenton RL, Maddie MA, Minnillo DR, Hagg T, Whittemore SR
JournalJ Comp Neurol
PubMed ID18092342
'After traumatic spinal cord injury (SCI), disruption and plasticity of the microvasculature within injured spinal tissue contribute to the pathological cascades associated with the evolution of both primary and secondary injury. Conversely, preserved vascular function most likely results in tissue sparing and subsequent functional recovery. It has been difficult to ... More
Differential effects of flavonoids on barrier integrity in human intestinal caco-2 cells.
AuthorsNoda S, Tanabe S, Suzuki T
JournalJ Agric Food Chem
PubMed ID22506771
'Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with ... More
The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex.
AuthorsMertens AE, Rygiel TP, Olivo C, van der Kammen R, Collard JG,
JournalJ Cell Biol
PubMed ID16186252
'The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) ... More
Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium.
AuthorsAcharya P, Beckel J, Ruiz WG, Wang E, Rojas R, Birder L, Apodaca G,
JournalAm J Physiol Renal Physiol
PubMed ID15068973
'In mammals, the bladder stores urine without permitting the passage of urine contents into the bloodstream, a function, in part, of the uroepithelial-associated tight junction complex. The protein constituents that make up this high-resistance barrier in the bladder are currently unknown, although the claudins, a multigene family, are thought to ... More
The Hedgehog pathway promotes blood-brain barrier integrity and CNS immune quiescence.
AuthorsAlvarez JI, Dodelet-Devillers A, Kebir H, Ifergan I, Fabre PJ, Terouz S, Sabbagh M, Wosik K, Bourbonnière L, Bernard M, van Horssen J, de Vries HE, Charron F, Prat A
JournalScience
PubMed ID22144466
'The blood-brain barrier (BBB) is composed of tightly bound endothelial cells (ECs) and perivascular astrocytes that regulate central nervous system (CNS) homeostasis. We showed that astrocytes secrete Sonic hedgehog and that BBB ECs express Hedgehog (Hh) receptors, which together promote BBB formation and integrity during embryonic development and adulthood. Using ... More
Cyclooxygenase-2 deficiency leads to intestinal barrier dysfunction and increased mortality during polymicrobial sepsis.
AuthorsFredenburgh LE, Velandia MM, Ma J, Olszak T, Cernadas M, Englert JA, Chung SW, Liu X, Begay C, Padera RF, Blumberg RS, Walsh SR, Baron RM, Perrella MA
JournalJ Immunol
PubMed ID21967897
'Sepsis remains the leading cause of death in critically ill patients, despite modern advances in critical care. Intestinal barrier dysfunction may lead to secondary bacterial translocation and the development of the multiple organ dysfunction syndrome during sepsis. Cyclooxygenase (COX)-2 is highly upregulated in the intestine during sepsis, and we hypothesized ... More
Interaction of zonula occludens-1 (ZO-1) with alpha-actinin-4: application of functional proteomics for identification of PDZ domain-associated proteins.
AuthorsChen VC, Li X, Perreault H, Nagy JI,
JournalJ Proteome Res
PubMed ID16944923
The use of recombinant
Invasive behavior of ulcerative colitis-associated carcinoma is related to reduced expression of CD44 extracellular domain: comparison with sporadic colon carcinoma.
AuthorsMikami T, Yoshida T, Numata Y, Kikuchi M, Araki K, Nakada N, Okayasu I
JournalDiagn Pathol
PubMed ID21473743
To elucidate relations of invasion of ulcerative colitis (UC)-associated carcinoma with its prognosis, the characteristics of invasive fronts were analyzed in comparison with sporadic colonic carcinomas. ... More
Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier.
AuthorsXu H, Dawson R, Crane IJ, Liversidge J
JournalInvest Ophthalmol Vis Sci
PubMed ID15980240
Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial. ... More
The protein tyrosine phosphatase Pez is a major phosphatase of adherens junctions and dephosphorylates beta-catenin.
AuthorsWadham C, Gamble JR, Vadas MA, Khew-Goodall Y,
JournalMol Biol Cell
PubMed ID12808048
Cell-cell adhesion regulates processes important in embryonal development, normal physiology, and cancer progression. It is regulated by various mechanisms including tyrosine phosphorylation. We have previously shown that the protein tyrosine phosphatase Pez is concentrated at intercellular junctions in confluent, quiescent monolayers but is nuclear in cells lacking cell-cell contacts. We ... More
Perivascular-resident macrophage-like melanocytes in the inner ear are essential for the integrity of the intrastrial fluid-blood barrier.
AuthorsZhang W, Dai M, Fridberger A, Hassan A, Degagne J, Neng L, Zhang F, He W, Ren T, Trune D, Auer M, Shi X,
JournalProc Natl Acad Sci U S A
PubMed ID22689949
The microenvironment of the cochlea is maintained by the barrier between the systemic circulation and the fluids inside the stria vascularis. However, the mechanisms that control the permeability of the intrastrial fluid-blood barrier remain largely unknown. The barrier comprises endothelial cells connected to each other by tight junctions and an ... More
Examination of the restoration of epithelial barrier function following superficial keratectomy.
AuthorsHutcheon AE, Sippel KC, Zieske JD,
JournalExp Eye Res
PubMed ID17067576
The goal of the present study was to determine the rate of restoration of the corneal epithelial barrier following a superficial keratectomy using a functional assay of tight junction integrity. Adult Sprague-Dawley rats were anesthetized and a 3-mm superficial keratectomy was performed. The eyes were allowed to heal from 4 ... More
The specific fates of tight junction proteins in apoptotic epithelial cells.
AuthorsBojarski C, Weiske J, Schöneberg T, Schröder W, Mankertz J, Schulzke JD, Florian P, Fromm M, Tauber R, Huber O,
JournalJ Cell Sci
PubMed ID15054114
The polarized morphology of epithelial cells depends on the establishment and maintenance of characteristic intercellular junctions. The dramatic morphological changes observed in apoptotic epithelial cells were ascribed at least in part to the specific fragmentation of components of adherens junctions and desmosomes. Little, however, is known about tight junctions during ... More
Occludin confers adhesiveness when expressed in fibroblasts.
AuthorsVan Itallie CM, Anderson JM,
JournalJ Cell Sci
PubMed ID9175707
Occludin is an integral membrane protein specifically associated with tight junctions. Previous studies suggest it is likely to function in forming the intercellular seal. In the present study, we expressed occludin under an inducible promotor in occludin-null fibroblasts to determine whether this protein confers intercellular adhesion. When human occludin is ... More
Epidermal growth factor receptor activation differentially regulates claudin expression and enhances transepithelial resistance in Madin-Darby canine kidney cells.
AuthorsSingh AB, Harris RC,
JournalJ Biol Chem
PubMed ID14593119
Tight junctions (TJs) are the most apical cell-cell junctions, and claudins, the recently identified TJ proteins, are critical for maintaining cell-cell adhesion in epithelial cell sheets. Based on their in vivo distribution and the results of overexpression studies, certain claudins, including claudin-1 and -4, are postulated to increase, whereas other ... More
Induction by transforming growth factor-beta1 of epithelial to mesenchymal transition is a rare event in vitro.
AuthorsBrown KA, Aakre ME, Gorska AE, Price JO, Eltom SE, Pietenpol JA, Moses HL,
JournalBreast Cancer Res
PubMed ID15084245
Transforming growth factor (TGF)-beta1 is proposed to inhibit the growth of epithelial cells in early tumorigenesis, and to promote tumor cell motility and invasion in the later stages of carcinogenesis through the induction of an epithelial to mesenchymal transition (EMT). EMT is a multistep process that is characterized by changes ... More
Inhibition of the Raf/MEK/ERK pathway up-regulates expression of the coxsackievirus and adenovirus receptor in cancer cells.
AuthorsAnders M, Christian C, McMahon M, McCormick F, Korn WM,
JournalCancer Res
PubMed ID12727824
Recombinant adenoviruses are presently being tested clinically as a new strategy for the treatment of cancer. An important determining factor for the successful entry of such adenoviruses into target cells is expression of the coxsackievirus and adenovirus receptor (CAR) at the cell surface. Recent observations suggest that expression of this ... More
Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells.
AuthorsIkari A, Tonegawa C, Sanada A, Kimura T, Sakai H, Hayashi H, Hasegawa H, Yamaguchi M, Yamazaki Y, Endo S, Matsunaga T, Sugatani J,
Journal
PubMed ID24659781
Claudin-16 (CLDN16) regulates the paracellular reabsorption of Mg(2+) in the thick ascending limb (TAL) of Henle's loop. However, the mechanism regulating the tight junctional localization of CLDN16 remains unknown. In yeast two-hybrid systems, we found that CLDN16 bound to syntaxin 8 (STX8), a target soluble N-ethylmaleimide-sensitive factor attachment protein receptor. ... More
Embolus extravasation is an alternative mechanism for cerebral microvascular recanalization.
AuthorsLam CK, Yoo T, Hiner B, Liu Z, Grutzendler J,
JournalNature
PubMed ID20505729
Cerebral microvascular occlusion is a common phenomenon throughout life that might require greater recognition as a mechanism of brain pathology. Failure to recanalize microvessels promptly may lead to the disruption of brain circuits and significant functional deficits. Haemodynamic forces and the fibrinolytic system are considered to be the principal mechanisms ... More
Connexin43 associated with an N-cadherin-containing multiprotein complex is required for gap junction formation in NIH3T3 cells.
AuthorsWei CJ, Francis R, Xu X, Lo CW,
JournalJ Biol Chem
PubMed ID15741167
Previous studies have indicated an intimate linkage between gap junction and adherens junction formation. It was suggested this could reflect the close membrane-membrane apposition required for junction formation. In NIH3T3 cells, we observed the colocalization of connexin43 (Cx43alpha1) gap junction protein with N-cadherin, p120, and other N-cadherin-associated proteins at regions ... More
Isoforms of the polarity protein par6 have distinct functions.
AuthorsGao L, Macara IG,
JournalJ Biol Chem
PubMed ID15292221
PAR-6 is essential for asymmetric division of the Caenorhabditis elegans zygote. It is also critical for cell polarization in many other contexts throughout the Metazoa. The Par6 protein contains a PDZ domain and a partial CRIB (Cdc42/Rac interactive binding) domain, which mediate interactions with other polarity proteins such as Par3, ... More
Differential distribution of tight junction proteins suggests a role for tanycytes in blood-hypothalamus barrier regulation in the adult mouse brain.
AuthorsMullier A, Bouret SG, Prevot V, Dehouck B,
JournalJ Comp Neurol
PubMed ID20127760
The median eminence is one of the seven so-called circumventricular organs. It is located in the basal hypothalamus, ventral to the third ventricle and adjacent to the arcuate nucleus. This structure characteristically contains a rich capillary plexus and features a fenestrated endothelium, making it a direct target of blood-borne molecules. ... More
Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1.
AuthorsFang X, Neyrinck AP, Matthay MA, Lee JW,
JournalJ Biol Chem
PubMed ID20554518
Acute lung injury is characterized by injury to the lung epithelium that leads to impaired resolution of pulmonary edema and also facilitates accumulation of protein-rich edema fluid and inflammatory cells in the distal airspaces of the lung. Recent in vivo and in vitro studies suggest that mesenchymal stem cells (MSC) ... More
Regulation of the blood-testis barrier by coxsackievirus and adenovirus receptor.
AuthorsSu L, Mruk DD, Cheng CY,
JournalAm J Physiol Cell Physiol
PubMed ID22875787
The blood-testis barrier (BTB) divides the seminiferous epithelium into the basal and the adluminal compartment. It restricts paracellular diffusion of molecules between Sertoli cells, confers cell polarity, and creates a unique microenvironment in the adluminal compartment for spermatid development. However, it undergoes restructuring during the epithelial cycle so that preleptotene ... More
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
AuthorsGuillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S,
JournalJ Cell Sci
PubMed ID22946046
Cingulin (CGN) is a 140 kDa protein, which is localized to the cytoplasmic region of vertebrate tight junctions (TJ), and regulates gene expression and RhoA signaling in cultured cells. To investigate the function of CGN at the organism level, we generated CGN knockout (CGN(-/-)) mice by homologous recombination. CGN(-/-) mice ... More
Identification of PKCzetaII: an endogenous inhibitor of cell polarity.
AuthorsParkinson SJ, Le Good JA, Whelan RD, Whitehead P, Parker PJ
JournalEMBO J
PubMed ID14685273
A new member of the atypical protein kinase C (aPKC) family, designated PKCzetaII, is identified in this study. The gene contains no introns and is 98% homologous with the cDNA encoding PKCzeta. The PKCzetaII coding region is frame-shifted with respect to the PKCzeta open reading frame, resulting in expression of ... More
Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin.
AuthorsCordenonsi M, D'Atri F, Hammar E, Parry DA, Kendrick-Jones J, Shore D, Citi S
JournalJ Cell Biol
PubMed ID10613913
We characterized the sequence and protein interactions of cingulin, an M(r) 140-160-kD phosphoprotein localized on the cytoplasmic surface of epithelial tight junctions (TJ). The derived amino acid sequence of a full-length Xenopus laevis cingulin cDNA shows globular head (residues 1-439) and tail (1,326-1,368) domains and a central alpha-helical rod domain ... More
Drebrin, an actin-binding, cell-type characteristic protein: induction and localization in epithelial skin tumors and cultured keratinocytes.
AuthorsPeitsch WK, Hofmann I, Bulkescher J, Hergt M, Spring H, Bleyl U, Goerdt S, Franke WW
JournalJ Invest Dermatol
PubMed ID16185277
Isoform E2 of drebrin, an actin-binding protein originally identified in neuronal cells, has recently been identified in diverse non-neuronal cells, mostly in association with cell processes and intercellular junctions. Here, we report on the presence of drebrin in normal human skin, epithelial skin cancers, and cultured keratinocytes. Keratinocytes of normal ... More
The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions.
AuthorsWillott E, Balda MS, Fanning AS, Jameson B, Van Itallie C, Anderson JM
JournalProc Natl Acad Sci U S A
PubMed ID8395056
Tight junctions form an intercellular barrier between epithelial cells, serve to separate tissue compartments, and maintain cellular polarity. Paracellular sealing properties vary among cell types and are regulated by undefined mechanisms. Sequence of the full-length cDNA for human ZO-1, the first identified tight junction component, predicts a protein of 1736 ... More
Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells.
AuthorsPoliak S, Matlis S, Ullmer C, Scherer SS, Peles E
JournalJ Cell Biol
PubMed ID12403818
The apposed membranes of myelinating Schwann cells are joined by several types of junctional specializations known as autotypic or reflexive junctions. These include tight, gap, and adherens junctions, all of which are found in regions of noncompact myelin: the paranodal loops, incisures of Schmidt-Lanterman, and mesaxons. The molecular components of ... More
Two classes of tight junctions are revealed by ZO-1 isoforms.
AuthorsBalda MS, Anderson JM
JournalAm J Physiol
PubMed ID7682777
The tight junction forms the intercellular barrier separating tissue compartments. The characteristics of this barrier are remarkably diverse among different epithelia and endothelia and are not explained by our limited knowledge of its molecular composition. Two isoforms of the 220-kDa tight junction protein ZO-1 result from alternative RNA splicing and ... More
ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin.
A 130-kD protein that coimmunoprecipitates with the tight junction protein ZO-1 was bulk purified from Madin-Darby canine kidney (MDCK) cells and subjected to partial endopeptidase digestion and amino acid sequencing. A resulting 19-amino acid sequence provided the basis for screening canine cDNA libraries. Five overlapping clones contained a single open ... More
Two strikingly different signaling pathways are induced by meningococcal type IV pili on endothelial and epithelial cells.
AuthorsLécuyer H, Nassif X, Coureuil M
JournalInfect Immun
PubMed ID22064711
Following adhesion on brain microvasculature, Neisseria meningitidis is able to cross the blood-brain barrier (BBB) by recruiting the polarity complex and the cell junction proteins, thus allowing the opening of the paracellular route. This feature is the consequence of the activation by the type IV pili of the ß2-adrenergic receptor/ß-arrestin ... More
Expression of zonula occludens-1 (ZO-1) and the transcription factor ZO-1-associated nucleic acid-binding protein (ZONAB)-MsY3 in glial cells and colocalization at oligodendrocyte and astrocyte gap junctions in mouse brain.
AuthorsPenes MC, Li X, Nagy JI
JournalEur J Neurosci
PubMed ID16045494
The PDZ domain-containing protein zonula occludens-1 (ZO-1) interacts with several members of the connexin (Cx) family of gap junction-forming proteins and has been localized to gap junctions, including those containing Cx47 in oligodendrocytes. We now provide evidence for ZO-1 expression in astrocytes in vivo and association with astrocytic connexins by ... More
14-3-3s and p63 play opposing roles in epidermal tumorigenesis.
AuthorsLi Q, Sambandam SA, Lu HJ, Thomson A, Kim SH, Lu H, Xin Y, Lu Q
JournalCarcinogenesis
PubMed ID21926108
14-3-3s plays a regulatory role in epidermal epithelial differentiation and loss of 14-3-3s leads to increased proliferation and impaired differentiation. A tumor suppressor function for 14-3-3s has been proposed based on the fact that some epithelial-derived tumors lose 14-3-3s expression. p63, a p53 family member, is a master regulator of ... More
Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells.
AuthorsChen Y, Lu Q, Schneeberger EE, Goodenough DA
JournalMol Biol Cell
PubMed ID10712504
In the Madin-Darby canine kidney epithelial cell line, the proteins occludin and ZO-1 are structural components of the tight junctions that seal the paracellular spaces between the cells and contribute to the epithelial barrier function. In Ras-transformed Madin-Darby canine kidney cells, occludin, claudin-1, and ZO-1 were absent from cell-cell contacts ... More
Enteropathogenic E. coli disrupts tight junction barrier function and structure in vivo.
AuthorsShifflett DE, Clayburgh DR, Koutsouris A, Turner JR, Hecht GA
JournalLab Invest
PubMed ID16127426
Enteropathogenic Escherichia coli (EPEC) infection disrupts tight junctions (TJs) and perturbs intestinal barrier function in vitro. E. coli secreted protein F (EspF) is, in large part, responsible for these physiological and morphological alterations. We recently reported that the C57BL/6J mouse is a valid in vivo model of EPEC infection as ... More