RPMI 1640 培养基、GlutaMAX™ 补充剂
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
RPMI 1640 培养基、GlutaMAX™ 补充剂
Gibco™

RPMI 1640 培养基、GlutaMAX™ 补充剂

RPMI 1640 培养基(Roswell Park Memorial Institute 1640 培养基)起初是为了悬浮培养人白血病单层细胞而开发的了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
61870036500 mL
6187012710 x 500 mL
货号 61870036
价格(CNY)
401.60
飞享价
Ends: 31-Dec-2025
509.00
共减 107.40 (21%)
Each
添加至购物车
数量:
500 mL
Customize this product
价格(CNY)
401.60
飞享价
Ends: 31-Dec-2025
509.00
共减 107.40 (21%)
Each
添加至购物车
RPMI 1640 培养基(Roswell Park Memorial Institute 1640 培养基)起初是为了悬浮培养人白血病单层细胞而开发的。RPMI 1640 培养基被发现适用于多种哺乳动物细胞,包括 HeLa、Jurkat、MCF-7、PC12、PBMC、星形胶质细胞和癌细胞。我们针对广泛的细胞培养应用,提供了多种 RPMI 1640 修饰。使用培养基选择工具查找适合的配方。

该 RPMI 1640 经过如下改良:
包含不含
•GlutaMAX™•HEPES:
• 酚红

可提供完整配方

使用 RPMI 1640 培养基
RPMI 1640 培养基相对于其他培养基而言较为独特,是因为其中含有还原剂谷胱甘肽和高浓度的维生素。RPMI 1640 培养基含有生物素、维生素 B12 以及 PABA,这些成分是 Eagle 最低必需培养基或 Dulbecco 改良 Eagle 培养基所不具备的。此外,该培养基之中还含有高浓度的维生素肌醇和胆碱。含有 GlutaMAX 补充剂的 RPMI 1640 培养基™ 可尽可能减少有毒氨的积聚,并可改善细胞活力和生长(易于使用的规格)。RPMI 1640 培养基不含蛋白、脂肪或生长因子。因此,RPMI 1640 培养基需要补充剂,通常采用 10% 胎牛血清 (FBS)。RPMI 1640 培养基使用碳酸氢钠缓冲系统 (2.0 g/L),因此需要 5–10% CO2 环境来维持生理 pH 值。

cGMP 生产和质量体系
RPMI 1640 培养基在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在 FDA 注册的医疗器械生产商,且通过 ISO 13485 标准认证。为确保供应链的稳定,我们可提供由苏格兰工厂生产的相同 RPMI 1640 产品 (61870-010)。该工厂也是在 FDA 注册的医疗器械生产商,且通过 ISO 13485 标准认证。
仅用于研究和生产用途。不可用于临床诊断或直接用于人类或动物。
规格
细胞系HeLa、Jurkat、MCF-7、PC-12、PBMC、星形胶质细胞和癌细胞
细胞类型白血病细胞
最大浓度1 X
生产质量cGMP-compliant under the ISO 13485 standard
产品线Gibco™、GlutaMAX™
产品类型RPMI 1640 培养基(Roswell Park Memorial Institute 1640 培养基)
数量500 mL
有效期自生产之日起 12 个月
运输条件室温
分类非动物源性
形式液体
血清水平标准血清
无菌无菌过滤
灭菌方法无菌过滤
加有添加剂GlutaMAX, 酚红
不加添加剂不含 HEPES, 不含丙酮酸钠
Unit SizeEach
内容与储存
储存条件:2-8°C。避光储存
运输条件:环境
有效期:自生产之日起 12 个月

常见问题解答 (FAQ)

清除,缺少或降解谷氨酰胺或生长因子等促生长的基本成份会对培养物的生长速率造成影响么?

是的。如果您怀疑发生了类似情况,请移去培养基并换用新的培养基。或者,您可以添加含有促生长成份的培养基。也可使用I或II型GlutaMAX取代培养基中的谷氨酰胺,以避免谷氨酰胺被耗竭。

 

配方中含有Gibco GlutaMAX添加剂会改变L-谷氨酰胺的含量么?

在所有以GlutaMAX双肽添加剂作为L-谷氨酰胺替代品的培养基中,谷氨酰胺的浓度都与原始配方中的L-谷氨酰胺浓度处于同等的摩尔水平。

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.