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Invitrogen
CD335 (NKp46) Monoclonal Antibody (9E2), Super Bright™ 436, eBioscience™
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图: 1 / 4
CD335 (NKp46) Antibody (62-3359-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (left) or Anti-Human CD335 (NKp46) Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.
产品信息
62-3359-42
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
9E2
偶联物
Super Bright™ 436
Super Bright™ 436
Super Bright™ 436
激发/发射光谱
413/431 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type I diabetes.
The 9E2 antibody has been reported to induce cytotoxicity.
Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 9E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
靶标信息
CD335, also known as NKp46 or NCR1, is a member of the natural cytotoxicity receptor family, which includes NKp30 and NKp44. This type I transmembrane glycoprotein is expressed on NK cells and plays a crucial role in activating and expanding resting NK cells through interactions with dendritic cells. CD335 enhances the ability of NK cells to eliminate tumor and virus-infected cells, such as those infected by influenza. CD335 consists of two Ig-like domains and is related to leukocyte immunoglobulin-like receptors (LIR) and killer inhibitory receptors (KIR). It contributes to the increased efficiency of activated NK cells in mediating tumor cell lysis. Additionally, CD335 interacts with pancreatic beta cells during the development of type 1 diabetes, highlighting its involvement in both immune defense and autoimmune processes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: 1-Mar; CD335; hNKp46; Ly-94; Lymphocyte antigen 94 homolog; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK cell-activating receptor; NK-p46
基因别名: CD335; LY94; NCR1; NK-p46; NKP46
UniProt ID: (Human) O76036
Entrez Gene ID: (Human) 9437
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