Substrate degradation by the proteasome: a single-molecule kinetic analysis.
AuthorsLu Y, Lee BH, King RW, Finley D, Kirschner MW,
Journal
PubMed ID25859050
'To address how the configuration of conjugated ubiquitins determines the recognition of substrates by the proteasome, we analyzed the degradation kinetics of substrates with chemically defined ubiquitin configurations. Contrary to the view that a tetraubiquitin chain is the minimal signal for efficient degradation, we find that distributing the ubiquitins as ... More