Restore™ Fluorescent Western Blot Stripping Buffer
Thermo Scientific™
Restore™ Fluorescent Western Blot Stripping Buffer
The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primaryRead more
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Catalog Number
Quantity
62299
20 mL
62300
100 mL
2 Options
Catalog number 62299
Price (CNY)
655.00
Each
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Quantity:
20 mL
Request bulk or custom format
Price (CNY)
655.00
Each
Add to cart
The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.
Features of Restore Fluorescent Western Blot Stripping Buffer:
• Fast—strip blots in only 15 minutes at room temperature • Saves time—no need to run new gels and prepare a new blot • Conserve samples—reprobe the same PVDF membrane for multiple targets • Economical—less expensive than other commercially available stripping buffers • Efficient—effectively strips blots the first time
Restore Fluorescent Western Blot Stripping Buffer enables the reuse of PVDF membranes, simplifying the Western blot optimization process and allowing the same blot to be reprobed with different primary antibodies to detect alternative targets. Restore Fluorescent Western Blot Stripping Buffer is for use with low-fluorescence PVDF membrane only (Part No. 22860).
Fluorescence Western blotting is a powerful method for detecting multiple targets at once. Restore Fluorescent Western Blot Stripping Buffer allows re-probing of PVDF membranes, saving time and cost. This is ideal when samples are limited and optimization or analysis with different primary antibodies is required.
Traditional stripping methods may adversely alter or remove the sample proteins from the PVDF membrane during the stripping process or may be effective for removing only low-affinity antibodies. In contrast, Restore Fluorescent Western Blot Stripping Buffer Stripping efficiency exceeds 90% while reprobing efficiency matches or exceeds other supplier's formulations. Also, Restore Fluorescent Western Blot Stripping Buffer is conveniently stored at room temperature and easy to use. Simply dilute buffer 1:5 in water and incubate your membrane for 5 to 20 minutes at room temperature.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
For Use With (Application)Western Blot
FormLiquid
Membrane CompatibilityPVDF
Product LineRestore™
Product TypeWestern Blot Stripping Buffer
Quantity20 mL
Unit SizeEach
Contents & Storage
Upon receipt store at room temperature.
Frequently asked questions (FAQs)
How many times can I strip my blot using Restore or Restore Plus Western Blot Stripping Buffers ?
The stability of the attached (transferred and bound) protein will determine the number of times the membrane can be successfully re-probed after stripping. The protein may withstand stripping as many as four times or as few as one time.
Can I strip membranes with Restore or Restore Plus Western Blot Stripping Buffer and reprobe for subsequent detection with fluorescent probes?
No. Although the fluorescent antibodies, like other antibodies, can be stripped with Restore Buffers, stripped membranes typically produce unacceptable background for subsequent fluorescent detection methods. We recommend Restore Fluorescent Western Blot Stripping Buffer, Cat. No. 62299 (20 mL) and Cat. No. 62300 (100 mL).
Note: Restore Fluorescent Western Blot Stripping Buffer is for use with low-fluorescence PVDF membrane (Cat. No. 22860) only.
Will the Restore and Restore Plus Western Blot Stripping Buffers remove precipitating substrates?
No. The antibodies are removed but the substrate leaves a permanent precipitate on the membrane that cannot be removed. Restore and Restore Plus Buffers are designed for procedures using chemiluminescent substrates. Please note that this is not compatible with fluorescence supstrates as it will result in increased background. For fluorescent substrates please use our Restore Fluorescent Western Blot Stripping Buffer. Please also see Tech Tip: Strip and Reprobe Western Blots (https://assets.thermofisher.com/TFS-Assets/BID/Technical-Notes/strip-reprobe-western-blots-tech-tip.pdf).
If I want to strip and re-probe a nitrocellulose membrane, what procedure do you recommend?
Thermo Fisher Scientific sells 4 ready-to-use buffers specifically intended for stripping Western blots prior to re-probing. Three of them can be used with both nitocellulose and PVDF membranes. We have Restore Western Blot Stripping Buffer (Cat. No. 21059), a trial size of this product (Cat. No. 21062), a product called Restore PLUS (Cat. No. 46428), which is formulated to strip off antibodies that are difficult to remove, and Restore Fluorescent Western Blot Stripping Buffer (Cat. No. 62299). The latter is used to strip fluorophore-labeled antibodies from PVDF membranes only.