Immuno 透明标准板

We recommend Frames and Accessories for Immuno Breakable Modules, BreakApart frame (Cat. No. 465404). This frame is designed to hold the Immuno Clear Standard Modules.

Our passive immunodiagnostic surfaces are based on a series of treatments that modify the polystyrene surface to different final levels of hydrophilicity. Selection of one of these surfaces for ELISA development should be based on the characteristics of the biomolecule you seek to immobilize. PolySorp is fairly hydrophobic and should be used for biomolecules that have hydrophobic domains, e.g., lipids, lipoproteins, and large proteins. MediSorp is slightly hydrophilic and can be used for biomolecules with hydrophilic and hydrophobic properties, e.g., medium-to-large proteins such as albumin, or amphiphilic biomolecules such as LPS. MaxiSorp is quite hydrophilic, optimized for binding of IgG (and any other molecule with similar characteristics) and thus is a popular choice for sandwich ELISA and similar protocols. MultiSorp is very hydrophilic, and in turn will immobilize very hydrophilic biomolecules such as glycoproteins. Some optimization will always be necessary, and in many cases screening for binding optimization may involve a few surface types.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

There is no difference in the manufacturing process. Certified plates are performance tested (using an antibody binding protocol) during quality control, whereas uncertified plates are not. This test is expensive for us to perform, so we offer the uncertified version at cost savings customers for whom antibody binding testing results are not critical.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.