Slide-A-Lyzer™ 透析盒，10K MWCO
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The original Slide-A-Lyzer™ Dialysis Cassettes will be discontinued once remaining inventory sells out. The next generation of Slide-A-Lyzer™ G3 Dialysis Cassettes offer improved ease of use and are the recommended replacement.
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Slide-A-Lyzer™ 透析盒，10K MWCO
引用和文献 (8)
Thermo Scientific™

Slide-A-Lyzer™ 透析盒，10K MWCO

Thermo Scientific Slide-A-Lyzer 透析盒 (10K MWCO) 有助于简单且有效地从大于 10,000 道尔顿的蛋白和其他大分子中去除缓冲盐和小分子污染物。Slide-A-Lyzer了解更多信息
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货号样品体积（公制）数量产品类型
663803 mL10盒透析盒
663830.5 mL10盒透析盒
663840.5 mL5 x 10 透析盒透析盒
663850.5 mL10盒透析盒套装
663813 mL5 x 10 透析盒透析盒
663823 mL10盒透析盒套装
6681012 mL8盒透析盒
6681112 mL5 x 8 透析盒透析盒
6680712 mL8盒透析盒套装
6683030 mL6盒透析盒
664540.5 mL10盒透析盒（γ 射线辐照）
664553 mL10盒透析盒（γ 射线辐照）
6645312 mL8盒透析盒（γ 射线辐照）
6645630 mL6盒透析盒（γ 射线辐照）
货号 66380
价格（CNY)
1,526.00
Each
添加至购物车
样品体积（公制）:
3 mL
数量:
10盒
产品类型:
透析盒
请求批量或定制报价
价格（CNY)
1,526.00
Each
添加至购物车
Thermo Scientific Slide-A-Lyzer 透析盒 (10K MWCO) 有助于简单且有效地从大于 10,000 道尔顿的蛋白和其他大分子中去除缓冲盐和小分子污染物。

Slide-A-Lyzer 透析盒特点：

•易于使用—无需打结或夹具；只需将样品注入盒中，即可开始透析
• 快速透析—带有两膜的平盒腔室提供了较高的表面积与体积之比，与圆柱形透析管相比，可极大程度地提高扩散率
• 高回收率—矩形透析盒设计通过四个角的任一进样口极大程度地提高整个样品体积的回收率
• 四种透析盒尺寸—选择较适合您样品体积的透析盒
• 颜色编码—橙色的透析盒框架指定了配有 10,000 MW 孔径膜的透析盒
• 经 γ 射线辐照选项—经 γ 射线辐照透析盒适用于要求尽可能减少样品污染风险的应用

10K 透析膜规格：
•组成：以纤维胶方式合成的再生纤维素
• 厚度：0.9 至 1.2 mil（22.5 至 30 µM）
•使用前要求进行的水合时间：小体积样品需 30 秒
•甘油含量：21%
•硫含量：0.05%
• 重金属含量：痕量

12 mL 规格方便用于制备应用
Slide-A-Lyzer 透析盒有四种不同规格可供选择，可容纳 0.1 至 30 mL 的样品体积。与传统透析管相比，这些装置更易于使用，且可提供更快的缓冲液更换和更完整的样品回收。Slide-A-Lyzer透析盒可用于各种应用，包括低分子量污染物去除、缓冲液更换、脱盐、平衡透析和样本浓缩。可用注射器上提供的皮下注射用针头穿过自密封衬垫，很容易地加入或抽出液体样本。在透析过程中，无需打结、夹具或盖帽，即可密封设备并防止泄漏和样品丢失。

伽马辐射透析盒：
伽马射线辐射的 Slide-A-Lyzer 透析盒非常适合研究人员培养细胞和微生物；纯化病毒、DNA 和 RNA；或在其他要求尽可能减少样品污染风险的应用中进行样品制备。经 γ 射线辐照透析盒经过 20-36 kGy γ 射线照射处理，这一装置灭菌剂量是根据用于确定总生长的胰酪大豆胨液体培养基和用于确定厌氧生长的液体硫乙醇酸盐培养基确定的。但是，由于没有在辐照后作进一步的无菌测试，因此未提供单个批次的无菌确认信息。

Slide-A-Lyzer 透析盒由两片低结合力的再生纤维素透析膜构成，该膜在惰性塑料框架内的硅胶状垫片的两侧均气密密封。膜和透析盒材料与大多数常见实验室化学品和缓冲液兼容。

更多产品数据
• 透析膜分离特点

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Slide-A-Lyzer™ 透析盒，10K MWCO，30 mL
For Research Use Only. Not for use in diagnostic procedures.
规格
膜纤维素膜，透析
产品线Slide-A-Lyzer
产品类型透析盒
纯化目标缓冲液置换，蛋白质
数量10盒
产品规格试剂盒、透析盒
截留分子量 (MWCO)10 kDa
样品体积（公制）3 mL
Unit SizeEach
内容与储存
室温保存

常见问题解答 (FAQ)

Does the Slide-A-Lyzer Dialysis Cassette leak?

The innovative, patented design does not permit “wicking” that can occur with homemade microdialysis devices.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What sizes are available for the Slide-A-Lyzer Dialysis Cassette? What MWCO membranes are available?

Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K). The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What prevents the Slide-A-Lyzer Dialysis Cassette from leaking?

An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:

1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the purpose of the buoy for the Slide-A-Lyzer Dialysis Cassette?

The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What is the highest temperature and the pH range that is compatible with the Slide-A-Lyzer Cassette?

Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all Slide-A-Lyzer™ 透析盒，10K MWCO FAQs

引用和文献 (8)

引用和文献
Abstract
Tyrosine-Based Cross-Linking of Peptide Antigens to Generate Nanoclusters with Enhanced Immunogenicity: Demonstration Using the Conserved M2e Peptide of Influenza A.
Authors:Wilks LR,Joshi G,Grisham MR,Gill HS
Journal:ACS infectious diseases
PubMed ID:34432416
A method of creating nanoclusters (NCs) from soluble peptide molecules is described utilizing an approach based on a tyrosine-tyrosine cross-linking reaction. A reactive tag comprising histidine and tyrosine residues was introduced at the termini of the peptide molecules. The cross-linking reaction led to the creation of dityrosine bonds within the ... More
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.
Authors:Otomo K,Omura T,Nozawa Y,Edwards SJ,Sato Y,Saito Y,Yagishita S,Uchida H,Watakabe Y,Naitou K,Yanai R,Sahara N,Takagi S,Katayama R,Iwata Y,Shiokawa T,Hayakawa Y,Otsuka K,Watanabe-Takano H,Haneda Y,Fukuhara S,Fujiwara M,Nii T,Meno C,Takeshita N,Yashiro K,Rosales Rocabado JM,Kaku M,Yamada T,Oishi Y,Koike H,Cheng Y,Sekine K,Koga JI,Sugiyama K,Kimura K,Karube F,Kim H,Manabe I,Nemoto T,Tainaka K,Hamada A,Brismar H,Susaki EA
Journal:Nature communications
PubMed ID:38866781
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution ... More
Determination of seroprevalence and kinetics of humoral response using mpox virus A29 protein.
Authors:Cai JP,Chu WM,Tam AR,Wang K,Han Y,Chen LL,Zhang X,Choi CY,Cheng VC,Chan KH,Chen Z,Hung IF,Fong CH,To KK
Journal:Communications medicine
PubMed ID:37993690
BACKGROUND: Mpox virus (MPXV), previously known as monkeypox virus, has spread globally in 2022. An accurate and convenient antibody test is essential for the determination of seroprevalence and for studying immune response after natural infection or vaccination. Most seroprevalence or vaccine studies used either live MPXV (or vaccinia virus [VACV]) or ... More
ADIBO-based "click" chemistry for diagnostic peptide micro-array fabrication: physicochemical and assay characteristics.
Authors:Prim D,Rebeaud F,Cosandey V,Marti R,Passeraub P,Pfeifer ME
Journal:Molecules (Basel, Switzerland)
PubMed ID:23959194
Several azide-derivatized and fluorescently-labeled peptides were immobilized on azadibenzocyclooctyne (ADIBO)-activated slide surfaces via a strain-promoted alkyne-azide cycloaddition (SPAAC) reaction revealing excellent immobilization kinetics, good spot homogeneities and reproducible fluorescence signal intensities. A myc-peptide micro-array immunoassay showed an antibody limit-of-detection (LOD) superior to a microtiter plate-based ELISA. Bovine serum albumin (BSA) ... More
Development of a high-throughput enzyme-linked immunosorbent assay for the routine detection of the carcinogen acrylamide in food, via rapid derivatisation pre-analysis.
Authors:Preston A,Fodey T,Elliott C
Journal:Analytica chimica acta
PubMed ID:18215649
The spontaneous formation of the neurotoxic carcinogen acrylamide in a wide range of cooked foods has recently been discovered. These foods include bread and other bakery products, crisps, chips, breakfast cereals, and coffee. To date, the diminutive size of acrylamide (71.08 Da) has prevented the development of screening immunoassays for ... More
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