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View additional product information for Slide-A-Lyzer™ Accessory Float Buoys and Syringes - FAQs (66432, 66430, 66490, 66493, 66494)
11 product FAQs found
The innovative, patented design does not permit “wicking” that can occur with homemade microdialysis devices.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Four sizes to accommodate samples between 0.1 and 30 ml are currently available in five different MWCOs (2K, 3.5K, 7K, 10K and 20K).
The G2 Slide A lyzers on the other hand are available in five different sizes to accommodate samples between 0.25 and 70mL.
These units, especially those that accommodate smaller volumes, provide for greater sample recovery and shorter dialysis times than one can achieve with traditional tubing. We have a cassette selection guide to help you choose the appropriate cassette for your application. There are also product demonstrations for this and other High-Performance Dialysis Products.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
An internal gasket, which isn't visible after the cassette is assembled, reseals itself as the needle is withdrawn. To maintain sample volume and integrity, users should remember these three key rules:
1. Before use, fill the cassette with air to verify that the system is airtight.
2. Puncture each porthole only once.
3. Do not allow the membrane to come into contact with the sharp needle bevel.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The buoy suspends the Slide-A-Lyzer Cassette above the stirring bar during dialysis and it provides a benchtop stand on which to set the Slide-A-Lyzer Cassette. These are not required with the G2 Slide-A-Lyzer Dialysis Cassettes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Slide-A-Lyzer Cassettes can be held at 56 degrees C for two hours. At temperatures and times above these, the plastic gets soft and can deform. Thermo Scientific recommends that users maintain a pH range of 5-9.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Thermo Scientific researchers have successfully used 10% solutions of DMF, DMSO, acetonitrile, methanol, hexane and heptane; 100% acetic acid; and 70% ethanol with the Slide-A-Lyzer Cassette. The units are compatible with sample containing up to 75% acetonitrile and 1% TFA; however, Thermo Scientific recommends not dialyzing into a buffer that contains an acetonitrile concentration greater than 10%.
The casing is made of ABS plastic and the gasket is made from a non-leaching silicone-like material. The membrane is made from regenerated cellulose.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
No. The Slide-A-Lyzer Cassette membrane requires no pretreatment. Trace metals are present in all Slide-A-Lyzer Cassette membranes. The 7,000 MWCO membranes contain 13% glycerol, 10,000 MWCO membranes contain 12% glycerol and 10,000 MWCO membranes contain 21% glycerol. These items will be lost during the dialysis process. To remove these items before introducing the sample, dialyze against water or a buffer for 10-15 minutes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
No, any 18-gauge needle that is at least one-inch long and any syringe that is of sufficient volume will work.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
It is possible to add reagents to the Slide-A-Lyzer Dialysis Cassette at different times using different portholes, allow a reaction to occur, and then dialyze in the same Slide-A-Lyzer Dialysis Cassette, reducing sample loss through reduced sample handling.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The Slide-A-Lyzer Cassette membrane is made of the same regenerated cellulose that is used in dialysis tubing, so a researcher should expect about the same protein loss one would experience with tubing. As a guide, a 1 mg/mL solution will generally have a recovery rate of greater than 95%. At 100µg/mL, the recovery rate drops to 75%-80%. With a dilute (e.g., 10 µg/mL) protein solution, one can lose up to 50% or more of the sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Typically, one should dialyze for one hour at room temperature three times in dialysate that is about 300 times the sample volume. If required, dialysis may continue overnight at 4 degrees C to yield a more complete dialysis. Because each sample is different, the optimum time must be derived empirically.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.