Low Fluorescence PVDF Membranes
Low Fluorescence PVDF Membranes
Invitrogen™

Low Fluorescence PVDF Membranes

Invitrogen™ Low Fluorescence PVDF Membranes are optimized to provide exceptional performance in western blot, fluorescent total protein normalization, and dot blot applications.
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货号产品规格尺寸 (长 x 宽)足够用于
678001Sandwich8.3 x 7.3 cm20 Blots
678000Roll8.3 cm x 10 m120 Mini-gel or 74 Midi-gel Blots
678002Sandwich13.5 x 8.3 cm20 Blots
678003Sheet8.3 x 7.3 cm10 Blots
678004Sheet13.5 x 8.3 cm10 Blots
货号 678001
价格（CNY)
2,090.00
飞享价
Ends: 31-Dec-2025
3,245.00
共减 1,155.00 (36%)
Each
添加至购物车
产品规格:
Sandwich
尺寸 (长 x 宽):
8.3 x 7.3 cm
足够用于:
20 Blots
价格（CNY)
2,090.00
飞享价
Ends: 31-Dec-2025
3,245.00
共减 1,155.00 (36%)
Each
添加至购物车

Invitrogen Low Fluorescence PVDF membranes are specifically optimized to provide exceptional performance in western blot, fluorescent total protein normalization, and dot blot applications. These membranes maintain high protein binding characteristics while providing minimal auto-fluorescence across a wide range of excitation wavelengths (280–800 nm). As a result, Low Fluorescence PVDF membranes provide excellent multi-plexing capability and help achieve higher signal-to-noise ratios and improved detection limits across a broad spectrum of fluorescent dyes.

Features and benefits

  • Low background autofluorescence—reduced background noise across multiple wavelengths, providing exceptional signal-to-noise
  • Consistency and reproducibility—optimized pore size engineered to produce exceptional results, regardless of protein size and abundance
  • Versatile formats—assortment of sizes and formats to match specific laboratory needs

Find the best format

Low Fluorescence PVDF membranes are available in rolls, pre-cut sheets, and pre-assembled membrane and filter paper sandwiches. The single-gel width roll can be easily cut to length for use with either mini or midi gel transfers. Precut sizes are available for both mini and midi gels, helping reduce handling effort and saving time. Compatible with wet tank transfer and semi-dry transfer systems, Low Fluorescence PVDF membranes pair with any preferred protein gel, helping improve data quality without changing the workflow.

Optimized pore size

Low Fluorescence PVDF membranes are manufactured with a narrower distribution of pore sizes compared to conventional PVDF membranes. The pore distribution of our 0.3 μm Low Fluorescence PVDF membranes is optimized to deliver consistent and reproducible results for western blotting applications, irrespective of target protein size. Low Fluorescence PVDF membranes facilitate uniform protein transfer and detection, helping ensure high-quality, reliable western blot data.

Applications

  • Fluorescent western blotting with IR and RGB fluorophores
  • Total protein normalization and quantitative western blotting
  • Chemiluminescent western blotting
For Research Use Only. Not for use in diagnostic procedures.
规格
适用于（应用）Western Blotting
产品类型Membrane/Filter Paper Sandwiches
数量20/Box
运输条件Room temperature
尺寸 (长 x 宽)8.3 x 7.3 cm
产品规格Sandwich
长度（公制）8.3 cm
材质PVDF
孔径0.3 μm
足够用于20 Blots
宽度（公制）7.3 cm
Unit SizeEach
内容与储存
Store at room temperature.

常见问题解答 (FAQ)

How long should I activate PVDF membrane in methanol?

We recommend wetting PVDF in 100% methanol or ethanol for 3 min and then rinsing with deionized (DI) water before use. Wetting for shorter times can result in incomplete activation, leading to inconsistent protein binding. Wetting for a longer time will not have a negative impact.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why does PVDF need to be activated?

PVDF is a hydrophobic membrane that will not readily interact or wet in water. To allow protein transfer and binding, the membrane must be initially wetted in methanol or ethanol and then rinsed with deionized (DI) water to allow protein binding to occur during transfer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

My PVDF membrane has dried out. What should I do?

If the PVDF membrane dries out after transfer and before immunodetection, the membrane can be re-wetted by soaking the membrane in 100% methanol or ethanol for 3 min and then rinsing with deionized (DI) water before proceeding to the blocking step. This re-wetting will generally not negatively impact protein binding.

If the PVDF membrane dries out before imaging, for fluorescent western blots, it can typically be imaged dry without re-wetting. However, note that some fluorescent dyes are prone to degradation which can accelerate when the membrane is dry. If wetting is desired, soak the membrane in 100% methanol or ethanol for 3 min and then rinse with DI water and proceed to imaging.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What are the recommended washing steps to ensure low background and high signal-to-noise ratio when using Invitrogen Low Fluorescence PVDF Membranes?

The recommended wash condition after each step is: TBS or PBS with 0.05% Tween 20 for 5 min, repeated 3 times.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can Invitrogen Low Fluorescence PVDF Membranes be stripped and re-probed, and if yes, how do I get the best results?

Yes. For best stripping and re-probing results, we recommend air drying the membrane after the transfer prior to immunoblotting (it will require rehydration in 100% methanol or ethanol prior to immunoblotting). Drying helps to fix the transferred proteins onto the membrane, helping ensure they remain immobilized and do not diffuse or wash away during subsequent steps. Drying the membrane can improve the binding efficiency of antibodies during the blocking and probing stages, resulting in better signal detection and stronger, clearer bands.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Low Fluorescence PVDF Membranes FAQs