White 96-Well Immuno Plates, Immulon, Round-bottom, Microfluor 1, 230μL - FAQs

Q: Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on top reader instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom. Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center .

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2 个常见问题解答

Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on “top reader” instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

Why would I use a black or white (instead of clear) immuno plate?

Black plates are often used in assays that make use of a fluorescent reporter because the opaque black surface effectively isolates the signal from each well in order to eliminate “crosstalk” or signal bleed. White plates also isolate well-to-well signals somewhat, but also provide a reflective background to direct luminescent output to the reader, and so often serve as a better choice to improve dynamic range from a luminescent reporter.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.