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View additional product information for Float Buoys, for 0.1 mL Slide-A-Lyzer™ MINI Dialysis Devices - FAQs (69588)
10 product FAQs found
The Slide-A-Lyzer MINI Dialysis Unit is compatible with alcohols, ketones, esters, oxides, and solvents containing nitrogen.
Alkalis: 1N sodium hydroxide or 1N ammonium hydroxide may cause pore swelling or shrinkage; not recommended to be used with 1N potassium hydroxide
Acids: Will survive weak acids (100% acetic or formic acids), or ~25% phosphoric or sulfuric acids; it should not be used with >25% strong acids, although a weak solution of a strong acid may work
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
In general, polypropylene and regenerated cellulose have very low nonspecific binding properties. The percent recovery of a sample will depend on the unique properties of the sample and on the initial concentration of the sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The membrane is regenerated cellulose and the plastic is a polypropylene copolymer. Both materials have very low protein and nucleic acid binding properties.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The Slide-A-Lyzer MINI Dialysis Unit can convert 100 µL of pH 2.8 buffer to pH 9.4 dialyzing against 1 liter bicarbonate buffer, pH 9.4 in less than 10 minutes.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
- Wearing gloves, apply sample (0.01-0.1 mL) using a pipette.
- OPTIONAL: Soak the Slide-A-Lyzer MINI Dialysis Unit to remove trace contaminants.
- Cap the Slide-A-Lyzer MINI Dialysis Unit (to minimize loss) and snap it into the float (sold separately).
- Insert the float into the beaker containing the dialysate.
- Recover sample.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
To remove glycerol, dialyze for 15 minutes against 1 liter of deionized water. Glycerol content is less than 3% in the 3K unit, approximately 15% in the 7K unit and 23% in the 10K unit. To remove metals, dialyze for 15 minutes against 1 liter of 1 mM EDTA. Metals present in the 3K, 7K and 10K unit include 2 ppb iron, 5 ppb magnesium, 1.5 ppb nickel, 0.2 ppb zinc, 0.2 ppb copper, 0.5 ppb chromium and 0.3 ppb cadmium. To remove sulfur, dialyze for 15 minutes against 1 liter of deionized water. Sulfur content should be minimal, less than 0.1%. Thermo Scientific quality control checks for sulfur on the membrane prior to the unit's assembly.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Always wear gloves when touching a Slide-A-Lyzer MINI Dialysis Unit If desired, soak the Slide-A-Lyzer MINI Dialysis Unit to remove contaminants For best recovery, tilt the tube and collect the sample in the bottom corner of the device.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
The Slide-A-Lyzer MINI Dialysis Unit is ideal for:
- Salt reduction Buffer exchange (<10 min for pH change 2.8 to 9.5)
- Removal of small molecular weight compounds
- Micro-conjugation (reagent addition or sample evaluation anytime)
- Equilibrium dialysis (microtube, paraffin to adjust depth)
- Concentration (in a microtube concentrates @ 35-45 µl/hr; use at least a 3:1 v/v ratio of concentrator to sample.)
- Sample preparation for instrumentation analysis (HPLC, CE, MS, GC, electrophoresis, etc.)
- Sample preparation for proteins, peptides, oligonucleotides, DNA, RNA (~330 MW/base ~660 MW/bp)
- The “scavenger” technique in which a resin, membrane, polymer or protein is placed in a microtube with buffer in order to select a compound to remove from a sample in a Slide-A-Lyzer MINI Dialysis Unit.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
Please view our selection table to choose the right dialysis device for your experiment - https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-purification-isolation/protein-dialysis-desalting-concentration/dialysis-products.html.
Dialysis is the separation of small and large molecules in a solution by selective diffusion through a semi-permeable membrane. It is generally used for larger sample volumes, and can take hours to overnight for complete dialysis.