Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton.
AuthorsXu K, Babcock HP, Zhuang X,
JournalNat Methods
PubMed ID22231642
By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained <10-nm lateral resolution and <20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. ... More