Are your PBS products tested for endotoxin levels?
Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Are your PBS products sterile and suitable for cell culture?
We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.
All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Are your PBS products DNase- and RNase-free?
We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Is a precipitate in PBS normal?
No, this is not normal and if there is a precipitate in the product, it cannot be used. If the precipitate will not go back into solution, please contact Technical Support at techsupport@thermofisher.com for a replacement.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
What is the difference between PBS and DPBS?
PBS has no potassium chloride (KCl) and it has a slightly lower level of phosphate (PO4) than DPBS. PBS is used for washing cells when viability isn't critical.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.