Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness.
AuthorsJami MS, Hou J, Liu M, Varney ML, Hassan H, Dong J, Geng L, Wang J, Yu F, Huang X, Peng H, Fu K, Li Y, Singh RK, Ding SJ,
Journal
PubMed ID24628760
KIAA1199 is a recently identified novel gene that is up-regulated in human cancer with poor survival. Our proteomic study on signaling polarity in chemotactic cells revealed KIAA1199 as a novel protein target that may be involved in cellular chemotaxis and motility. In the present study, we examined the functional significance ... More
Impact of diet deprivation and subsequent overallowance during gestation on mammary gland development and lactation performance.
AuthorsFarmer C, Palin MF, Martel-Kennes Y,
Journal
PubMed ID24352961
The impacts of diet deprivation and subsequent overallowance during gestation on mammary gene expression and development and lactation performance were determined. Gilts were reared under a conventional (control, CTL; n = 59) or an experimental (treatment, TRT; n = 56) dietary regimen during gestation. The experimental regimen provided 70% (restriction ... More
Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay.
Apoptosis can be detected reliably by assaying for cleaved caspase-3, for which active caspase-3 antibodies are used in several methods, such as immunocytochemistry, enzyme-linked immunosorbent assay, and western blot. In this study, we used TaqMan protein assay (TPA), a novel method for protein detection and quantification that detects proteins by ... More
Apoptosis-dependent acute lung injury and repair after intratracheal instillation of noradrenaline in rats.
AuthorsUhal BD, Rayford H, Zhuang J, Li X, Laukka J, Soledad-Conrad V
JournalExp Physiol
PubMed ID12621532
Earlier work in this laboratory showed that noradrenaline (NA) induces apoptosis in primary cultures of alveolar epithelial cells (AECs). Apoptosis of alveolar epithelial cells may promote the collapse of lung barrier function. On this basis we hypothesized that exogenous NA, administered by intratracheal (I.T.) instillation, might induce AEC apoptosis in ... More