Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization.
AuthorsAhmed MM, Gardiner KJ,
JournalJ Neurosci Methods
PubMed ID21236297
Post translational modification (PTM) and proteolytic processing of proteins contributes to regulation of their stability, intracellular localization and interactions with other proteins, and to direct enhancement or repression of their activity. Both PTM and proteolysis are dynamic; levels and rates change in response to changes in the cellular environment. Tissue ... More