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CCL5 (RANTES) Antibody (710001) in ICC/IF
Immunofluorescence analysis of CCL5/RANTES was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CCL5/RANTES (25HCLC) Recombinant Rabbit Superclonal™ Antibody (Product # 710001) at 2 µg/mL in 0.1% BSA and ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
710001
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
25HCLC
抗原
proprietary
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with rat and human based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; regulated upon activation normal T-cell expressed and secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell-specific protein RANTES; TCP228
基因别名: CCL5; D17S136E; MuRantes; RANTES; SCYA5; SISd; TCP228
UniProt ID: (Mouse) P30882
Entrez Gene ID: (Rat) 81780, (Mouse) 20304
phosphatidylinositol phospholipase C activity
protein kinase activity
chemokine activity
heparin binding
phospholipase activator activity
receptor signaling protein tyrosine kinase activator activity
CCR1 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
chemokine receptor binding
protein homodimerization activity
protein self-association
chemokine receptor antagonist activity
cytokine activity
cytokine
intercellular signal molecule
MAPK cascade
chronic inflammatory response
protein phosphorylation
calcium ion transport
cellular calcium ion homeostasis
exocytosis
inflammatory response
leukocyte cell-cell adhesion
G-protein coupled receptor signaling pathway
cell-cell signaling
aging
response to virus
response to bacterium
response to toxic substance
response to salt stress
positive regulation of gene expression
positive regulation of macrophage chemotaxis
positive regulation of T cell chemotaxis
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
response to activity
positive regulation of smooth muscle cell migration
dibenzo-p-dioxin metabolic process
positive regulation of cell migration
pseudopodium assembly
positive regulation of cellular biosynthetic process
activation of phospholipase D activity
positive regulation of fever generation
lipopolysaccharide-mediated signaling pathway
response to nutrient levels
response to lipopolysaccharide
response to insulin
positive regulation of cell-cell adhesion mediated by integrin
response to cytokine
positive regulation of homotypic cell-cell adhesion
response to interferon-gamma
response to tumor necrosis factor
T-helper 1 cell diapedesis
positive regulation of T cell proliferation
neutrophil activation
positive regulation of phosphorylation
response to drug
positive regulation of tyrosine phosphorylation of STAT protein
protein kinase B signaling
cellular protein complex assembly
response to estrogen
negative regulation by host of viral transcription
cellular response to fibroblast growth factor stimulus
negative regulation of viral genome replication
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
negative regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
positive regulation of cell adhesion
eosinophil chemotaxis
lymphocyte chemotaxis
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
regulation of insulin secretion
regulation of T cell activation
positive chemotaxis
protein tetramerization
response to glucocorticoid
positive regulation of calcium ion transport
negative regulation of cell death
positive regulation of mast cell chemotaxis
positive regulation of protein tyrosine kinase activity
chemokine-mediated signaling pathway
negative regulation of chemokine-mediated signaling pathway
negative regulation of T cell apoptotic process
positive regulation of T cell apoptotic process
response to cholesterol
cellular response to lipopolysaccharide
cellular response to amino acid stimulus
cellular response to vitamin K
cellular response to morphine
cellular response to cytokine stimulus
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to ethanol
cellular response to lipid
cellular response to high density lipoprotein particle stimulus
cellular response to organic cyclic compound
cellular response to alkyl hydroperoxide
cellular response to transforming growth factor beta stimulus
positive regulation of monocyte chemotaxis
regulation of neuron death
negative regulation of neuron death
negative regulation of macrophage apoptotic process
positive regulation of T cell migration
positive regulation of natural killer cell chemotaxis
positive regulation of defense response to virus by host
chemotaxis
immune response
extracellular negative regulation of signal transduction
negative regulation of receptor activity
positive regulation of chemokine (C-X-C motif) ligand 2 production
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