Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
AuthorsGovindarajan R, Zhao S, Song XH, Guo RJ, Wheelock M, Johnson KR, Mehta PP
JournalJ Biol Chem
PubMed ID12205082
'Gap junctions, composed of connexins, provide a pathway of direct intercellular communication for the diffusion of small molecules between cells. Evidence suggests that connexins act as tumor suppressors. We showed previously that expression of connexin-43 and connexin-32 in an indolent prostate cancer cell line, LNCaP, resulted in gap junction formation ... More
Immunogold evidence that neuronal gap junctions in adult rat brain and spinal cord contain connexin-36 but not connexin-32 or connexin-43.
AuthorsRash JE, Staines WA, Yasumura T, Patel D, Furman CS, Stelmack GL, Nagy JI,
JournalProc Natl Acad Sci U S A
PubMed ID10861019
Physiological and ultrastructural evidence indicates that gap junctions link many classes of neurons in mammalian central nervous system (CNS), allowing direct electrical and metabolic communication. Among at least six gap junction-forming connexin proteins in adult rat brain, connexin- (Cx) 32, Cx36, and Cx43 have been reported to occur in neurons. ... More
Connexin-47 and connexin-32 in gap junctions of oligodendrocyte somata, myelin sheaths, paranodal loops and Schmidt-Lanterman incisures: implications for ionic homeostasis and potassium siphoning.
AuthorsKamasawa N, Sik A, Morita M, Yasumura T, Davidson KG, Nagy JI, Rash JE,
JournalNeuroscience
PubMed ID16203097
The subcellular distributions and co-associations of the gap junction-forming proteins connexin 47 and connexin 32 were investigated in oligodendrocytes of adult mouse and rat CNS. By confocal immunofluorescence light microscopy, abundant connexin 47 was co-localized with astrocytic connexin 43 on oligodendrocyte somata, and along myelinated fibers, whereas connexin 32 without ... More
ECM-induced gap junctional communication enhances mammary epithelial cell differentiation.
The relationship between gap junctional intercellular communication (GJIC) and mammary cell (CID-9) differentiation in vitro was explored. CID-9 cells differentiate and express beta-casein in an extracellular matrix (ECM)- and hormone-dependent manner. In response to interaction with the ECM, cells in culture modulated the expression of their gap junction proteins at ... More
Role of gap junctions in fluid secretion of lacrimal glands.
AuthorsWalcott B, Moore LC, Birzgalis A, Claros N, Valiunas V, Ott T, Willecke K, Brink PR,
JournalAm J Physiol Cell Physiol
PubMed ID11832335
In glands such as the liver and pancreas, gap junctions containing connexin 26 and 32 (Cx26 and Cx32, respectively) couple the secretory cells. Uncoupling these junctions compromises the secretory function of these glands. Lacrimal glands also contain extensive arrays of gap junctions consisting of Cx26 and Cx32. We wanted to ... More
Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat.
Recent studies have suggested that cell-to-cell coupling, which occurs via gap junctions, may play a role in CO(2) chemoreception. Here, we used immunoblot and immunohistochemical analyses to investigate the presence, distribution, and cellular localization of the gap junction proteins connexin26 (Cx26) and connexin32 (Cx32) in putative CO(2)-chemosensitive brainstem regions in ... More