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IGF2BP1 Antibody (712138) in ICC/IF
For immunofluorescence analysis,HepG2 cells were fixed and permiabilized detection of endogenous IGF2BP1 using Anti-IGF2BP1 Recombinant Rabbit Superclonal™ Antibody (Product # 712138, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly ... View More
Product Details
712138
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
18HCLC
Immunogen
Peptides corresponding to human IGF2BP1 [1) aa162-aa178 2) aa561-aa577]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Dog, Cat.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
IGF2BP1 is a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: coding region determinant binding protein; Coding region determinant-binding protein; CRD-BP; IGF-II mRNA-binding protein 1; IGF2 mRNA-binding protein 1; insulin-like growth factor 2 mRNA binding protein 1 deltaN CRDBP; Insulin-like growth factor 2 mRNA-binding protein 1; insulin-like growth factor 2, binding protein 1; VICKZ family member 1; VIon ChannelKZ1; ZBP-1; zipcode binding protein 1; Zipcode-binding protein 1
Gene Aliases: AL024068; AW549074; CRD-BP; CRDBP; D030026A21Rik; D11Moh40e; D11Moh45; IGF2BP1; IMP-1; IMP1; mir-3063; Neilsen; VICKZ1; ZBP-1; ZBP1
UniProt ID: (Human) Q9NZI8, (Mouse) O88477
Entrez Gene ID: (Human) 10642, (Mouse) 140486
gene expression
regulation of mRNA stability involved in response to stress
negative regulation of translation
pallium cell proliferation in forebrain
regulation of cytokine biosynthetic process
mRNA transport
CRD-mediated mRNA stabilization
neuronal stem cell population maintenance
RNA localization
regulation of translation
transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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