Functional characterization and localization of a gill-specific claudin isoform in Atlantic salmon.
AuthorsEngelund MB, Yu AS, Li J, Madsen SS, Færgeman NJ, Tipsmark CK
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID21975646
'Claudins are the major determinants of paracellular epithelial permeability in multicellular organisms. In Atlantic salmon (Salmo salar L.), we previously found that mRNA expression of the abundant gill-specific claudin 30 decreases during seawater (SW) acclimation, suggesting that this claudin is associated with remodeling of the epithelium during salinity change. This ... More
CD44 regulates tight-junction assembly and barrier function.
AuthorsKirschner N, Haftek M, Niessen CM, Behne MJ, Furuse M, Moll I, Brandner JM,
JournalJ Invest Dermatol
PubMed ID21191420
'Upon barrier disturbance, adult CD44 knockout (KO) mice show delayed recovery of epidermal barrier function. This correlates with the loss of apical polarization of lamellar body (LB) secretion. As tight junctions (TJs) are crucial for barrier function and regulate polarized targeting of vesicles, we hypothesized that CD44 regulates TJs and ... More
Protection against hypoxia-induced increase in blood-brain barrier permeability: role of tight junction proteins and NFkappaB.
AuthorsBrown RC, Mark KS, Egleton RD, Huber JD, Burroughs AR, Davis TP,
JournalJ Cell Sci
PubMed ID12538770
'Co-culture with glial cells and glia-conditioned media can induce blood-brain barrier properties in microvessel endothelial cells and protect against hypoxia-induced blood-brain barrier breakdown. We examined the effect of two types of glia-conditioned media on brain microvessel endothelial cell permeability and tight junction protein expression, and studied potential mechanisms of action. ... More
The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction.
AuthorsNusrat A, Chen JA, Foley CS, Liang TW, Tom J, Cromwell M, Quan C, Mrsny RJ,
JournalJ Biol Chem
PubMed ID10887180
'Occludin is an integral membrane protein that has been suggested to play a role in the organization and dynamic function of the epithelial tight junction (TJ). A number of other proteins have also been described to localize to the TJ. We have used a novel bait peptide method to investigate ... More
Expression of claudin-1 and -11 in immature and mature pheasant (Phasianus colchicus) testes.
AuthorsPark CJ, Lee JE, Oh YS, Shim S, Nah WH, Choi KJ, Gye MC,
JournalTheriogenology
PubMed ID21074249
'The expression of claudin-1 and -11, tight junctions (TJs) proteins was examined in immature and adult pheasant (Phasianus colchicus) testes. Claudin-1 and -11 cDNA were highly similar to those of human, mice, and chicken. Claudin-1 mRNA and protein (21 kDa) levels in immature testes were higher than those of adult ... More
Differential effects of flavonoids on barrier integrity in human intestinal caco-2 cells.
AuthorsNoda S, Tanabe S, Suzuki T
JournalJ Agric Food Chem
PubMed ID22506771
'Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with ... More
Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier.
AuthorsXu H, Dawson R, Crane IJ, Liversidge J
JournalInvest Ophthalmol Vis Sci
PubMed ID15980240
Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permeability are controversial. ... More
Changes in the distribution pattern of Claudin tight junction proteins during the progression of mouse skin tumorigenesis.
AuthorsArabzadeh A, Troy TC, Turksen K,
JournalBMC Cancer
PubMed ID17945025
Despite the fact that morphological and physiological observations suggest that the tight junction (TJ)-based permeability barrier is modified/disrupted in tumorigenesis, the role of members of the Claudin (Cldn) family of TJ proteins is not well-understood. Using a well-established two-stage chemical carcinogenesis model, we investigated the temporal and spatial changes in ... More
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury.
AuthorsGuillemot L, Schneider Y, Brun P, Castagliuolo I, Pizzuti D, Martines D, Jond L, Bongiovanni M, Citi S,
JournalJ Cell Sci
PubMed ID22946046
Cingulin (CGN) is a 140 kDa protein, which is localized to the cytoplasmic region of vertebrate tight junctions (TJ), and regulates gene expression and RhoA signaling in cultured cells. To investigate the function of CGN at the organism level, we generated CGN knockout (CGN(-/-)) mice by homologous recombination. CGN(-/-) mice ... More
Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex.
AuthorsNunbhakdi-Craig V, Machleidt T, Ogris E, Bellotto D, White CL, Sontag E
JournalJ Cell Biol
PubMed ID12196510
Tight junctions (TJs) play a crucial role in the establishment of cell polarity and regulation of paracellular permeability in epithelia. Here, we show that upon calcium-induced junction biogenesis in Madin-Darby canine kidney cells, ABalphaC, a major protein phosphatase (PP)2A holoenzyme, is recruited to the apical membrane where it interacts with ... More
Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells.
AuthorsChen Y, Lu Q, Schneeberger EE, Goodenough DA
JournalMol Biol Cell
PubMed ID10712504
In the Madin-Darby canine kidney epithelial cell line, the proteins occludin and ZO-1 are structural components of the tight junctions that seal the paracellular spaces between the cells and contribute to the epithelial barrier function. In Ras-transformed Madin-Darby canine kidney cells, occludin, claudin-1, and ZO-1 were absent from cell-cell contacts ... More